BIOCONTROL OF PEA ROOT-ROT USING SELECTED MICROBIAL STRAINS | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 1, Volume 33, Issue 11, November 2008, Page 8091-8103 PDF (626.1 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2008.200732 | ||||
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| Authors | ||||
| Amal W. Abo El-Khair1; M.N.A. Khalil2; K.A. Abdel-Kawi1 | ||||
| 1Soils, Water and Environ. Res. Inst., Agric. Res. Center (ARC), Giza, Egypt. | ||||
| 2Plant Pathology Research Institute, ARC, Giza, Egypt. | ||||
| Abstract | ||||
| Fusarium solani, Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum, Macrophomina phseolina and Sclerotium rolfsii, were isolated from diseased pea root rot from different localities, i.e., Giza, Ismailia, Sharkiya, Qalubiya and Behiera Governorates. Results indicated that R. solani and F. solani were the most dominant in all Governorates. All the isolated fungi were pathogenic to pea plants, however, they varied in their virulence. In general, R. solani and F. solani were the most virulent. Some salts, bioagents and antioxidants were used as seed soaking as compared with the fungicidal Rizolex-T to control root-rot disease of pea, under greenhouse and field conditions. Results indicated that all the tested materials reduced pre- and post-emergence damping-off and increased the survival of plants as well as their yield and improved their quality (pod characteristics). These materials varied in their efficiency. The standard fungicide (Rhizolex-T) was the best in controlling the disease followed by acetyl salicylic acid (ASA), sodium salicylate (as antioxidant) and monopotassium sulphate (as salt), which gave good effects in controlling pea root rot disease as well as increased pea yield survival. Meantime, Pseudomonas fluorsecens and Bacillus subtilis (as bioagents) were moderately effective with Ca 64%, 78% and 75% for Serratia, Pseudomonas fluorsecens and Bacillus subtilis, respectively in comparison with 53% for control. On the other hand, calcium chloride (salt) and Serratia (bioagent) were the least effective ones. The best materials were considered non harmful control methods, which have advantage. Therefore, such materials should be used as effective and safe means for controlling soil-borne plant pathogens in order to avoid environmental pollution through decreasing chemical fungicides use. | ||||
| Keywords | ||||
| Pea root-rot; biocontrol; Bacillus subtilis; Pseudomonas fluorescens; Serratia | ||||
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