Genetic Characterization of Methicillin Resistance Staphylococcus aureus Isolates of Poultry and Human Origin
Saleh, M., El Hady, A., R. Mohamed, S., El-Shafei, A., El-Shafei, M. (2021). Genetic Characterization of Methicillin Resistance Staphylococcus aureus Isolates of Poultry and Human Origin. EKB Journal Management System, 52(Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021), 61-68. doi: 10.21608/ejvs.2021.95036.1292
Mohamed Saleh; A.M.M. El Hady; Sahar R. Mohamed; A. A. El-Shafei; Maher El-Shafei. "Genetic Characterization of Methicillin Resistance Staphylococcus aureus Isolates of Poultry and Human Origin". EKB Journal Management System, 52, Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021, 2021, 61-68. doi: 10.21608/ejvs.2021.95036.1292
Saleh, M., El Hady, A., R. Mohamed, S., El-Shafei, A., El-Shafei, M. (2021). 'Genetic Characterization of Methicillin Resistance Staphylococcus aureus Isolates of Poultry and Human Origin', EKB Journal Management System, 52(Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021), pp. 61-68. doi: 10.21608/ejvs.2021.95036.1292
Saleh, M., El Hady, A., R. Mohamed, S., El-Shafei, A., El-Shafei, M. Genetic Characterization of Methicillin Resistance Staphylococcus aureus Isolates of Poultry and Human Origin. EKB Journal Management System, 2021; 52(Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021): 61-68. doi: 10.21608/ejvs.2021.95036.1292

Genetic Characterization of Methicillin Resistance Staphylococcus aureus Isolates of Poultry and Human Origin

Egyptian Journal of Veterinary Sciences
Article 14, Volume 52, Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021, October 2021, Page 61-68  XML PDF (1.39 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2021.95036.1292
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Authors
Mohamed Saleh email 1; A.M.M. El Hady1; Sahar R. Mohamed1; A. A. El-Shafei1; Maher El-Shafei2
1Bacteriology Department , Animal Health Research Institute, Dokki , Giza, Egypt
2Department of Medical Microbiology Faculty of Medicine Zagazig University, Egypt
Abstract
Our study applied on 160 collected samples, from poultry and human patients. Infection percent of human skin, poultry bumble foot and human nasal swab and Diabetic foot swab were 66%  , 10% , 67.5% , 37.5%  respectively. Methicillin resistant Staph aureus (MRSA) were 83.3% and 50 % from poultry and human patient respectively, mec A gene is detected by 100% on samples of poultry and humen origin. Whereas blaZ and spa gene and also, 83.3% and 50% respectively either from human or poultry samples respectively. Methicillin Resistance S. aureus (MRSA) strains isolated from human nasal swabs was 80 % . PCR technique was used for mec A , blaZ ,and  Spa gene detection. PCR results were identical to disc diffusion method in 11 isolates out of 15 ( 73 %) isolates from poultry and human samples.

Keywords
Methicillin resistant; S.aureus; Human; Poultry; mec A gene; blaZ gene; spa gene
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