A Recent Non cytopathogenic bovine viral diarrhea virus detected by immunofluorescence and reverse transcriptase polymerase chain reaction | ||
| Benha Veterinary Medical Journal | ||
| Article 8, Volume 41, Issue 1, October 2021, Pages 39-41 PDF (345.18 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2021.79012.1427 | ||
| Authors | ||
| Samia Abdelkareem* 1; Ayman Said El-Habbaa2; zeinab R.A. aboezz3; Ehab ElNahas4 | ||
| 1Viral Diseases Research Unit, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), Giza, Egypt | ||
| 2Virology department, faculty of veterinary medicine, Benha university, Moshtohor, Egypt | ||
| 3Virology department, faculty of vet medicine, Benha university. | ||
| 4Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor 13736, Kalubia, Egypt | ||
| Abstract | ||
| In the present study, a non-cytopathogenic bovine viral diarrhea virus (BVDV) field strains from bovine semen samples were detected by immunofluorescence (IF) and reverse transcriptase polymerase chain reaction (RT-PCR). Two out of five semen samples tested positive for the presence of BVDV antigen employing antigen capture ELISA kits. The two semen samples were injected in Madin-Darby bovine kidney (MDBK) cells in a trial for BVDV isolation. The chosen two samples were not cytopathic and subjected for further identification and genotyping using one step reverse transcriptase polymerase chain reaction (RT-PCR) assay. The isolated BVDV field strains from semen were found to be BVDV genotype 1. | ||
| Keywords | ||
| Noncytopathic BVDV; Semen; MDBK; RT-PCR, Genotype1 | ||
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