Effect of treatment Osteosarcoma (Mg-63) cells with Vitamin C, Green tea and their combination (An-in vitro study) | ||
| Journal of Modern Research | ||
| Article 3, Volume 4, Issue 1, January 2022, Pages 27-31 PDF (755.16 K) | ||
| Document Type: Research Articles | ||
| DOI: 10.21608/jmr.2021.102961.1084 | ||
| Authors | ||
| Hiam Rifaat Hussien* 1; Amr Helmy Moustafa Elbolok2; Sherif Farouk Elgayar3; Maii Ibrahim Ali Sholqamy4 | ||
| 1Faculty of Dentistry, Minia University | ||
| 2Department of Oral Pathology, Faculty of Dentistry ,Minia University | ||
| 3Department of Oral Pathology ,Faculty of Dentistry , Minia University. | ||
| 4Department of Oral Pathology, Faculty of Dentistry , Minia University | ||
| Abstract | ||
| Abstract Background: Osteosarcoma (OS) is the most common primary bone malignancy that affects children and adolescents. It is considered as a serious threat to the human health globally. For many years plants and natural constitutes components such as vitamin C (ascorbic acid), green tea have been used as anti-cancer drugs. Aim: The objective of this in vitro study was to investigate the effect of vitamin C, green tea and their combination on Mg-63 cell line. Material Methods: Treatment of cell line (Mg-63) by different concentrations of vitamin, green tea and their combination was done to evaluate the viability of the treated cells by SRB assay. Microscopic examination and were applied. Results: Regarding cytotoxicity effect of vitamin C, green tea and their combination on Mg-63cell line, it was noticed that cell distribution showed a variable arrest at different phase of cell division. Where there was non-significant difference of arrested cells of these drugs compared with its value in non-treated G0-G1 phase control cells while there was a significant elevated arrest of treated cells during the G2-M phase and the significant difference of cell arrest at G2-M phase was type of treatment related. Conclusion: From the results of the present study, we noticed that vitamin green tea and their combination have cytotoxic effect on Mg-63 cell line, it also induced an effect on the cell cycle distribution, resulting in apoptosis, necrosis and autophagy. | ||
| Keywords | ||
| Key words Osteosarcoma (OS); vitamin C; green tea; combination and autophagy | ||
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