Molecular characterization of Salmonella serovars isolated from Chicken | ||
| Benha Veterinary Medical Journal | ||
| Article 20, Volume 41, Issue 1, October 2021, Pages 94-98 PDF (807.47 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2021.69331.1386 | ||
| Authors | ||
| Walaa Ragab Abd Elkader* 1; Ashraf Abd El Tawab2; Fatma El Hofy2; Aisha Ali3 | ||
| 1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine Benha University, Egypt. | ||
| 2Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University | ||
| 3Animal Health Research Institute, Serology Unit, Giza, Egypt | ||
| Abstract | ||
| A total of one hundred diseased chicken (100 samples of each of Liver , Gall bladder and Intestine content) suffering from diarrhea were collected from different broiler private farms in Giza governorate, Egypt. Samples were collected in a sterile container in an ice bag and then transferred aseptically to the laboratory for conventional bacteriological analysis Bacteriological examination revealed that Salmonella from liver were (2.66%), Gall bladder ( 3% ) and Intestine ( 1.66 % ). The prevalence rate to Salmonella was (7.66%). Antibiogram pattern was applied and isolates exhibit resistance against Rifampicin, Amoxycillin and Doxycyclin and highly sensitivity to Streptomycin with (100%) variable results were recorded with the remaining test antibiotic molecular characterizations of B-Lactamase resistance genes in salmonella serovars gave variable results. A PCR based assay was developed to detect the prevalence of Salmonella in samples and to and chromosome-borne virulence genes (blaTEM ,blaSHV and blaCTX ). The aims of the current study wasbacteriological and molecular studies on Salmonella species isolated from poultry farms | ||
| Keywords | ||
| Salmonella; B-Lactam; PCR | ||
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