Neuroprotective Effect of Piracetam and Vincamine in a Rat Model of Haloperidol-induced Parkinson's Disease | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 2, Volume 42, Issue 1, January 2022, Pages 11-26 PDF (610.92 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2021.71203.1099 | ||
| Authors | ||
| ALZAHRAA SHEREF* 1; YAHYA NAGUIB1; ELSAYED ABOUELNOUR2; HEBA SALEM1; MOHAMMED HASSAN1; HESHAM ABDEL-RAZEK1 | ||
| 1MEDICAL PHYSIOLOGY,FACULTY OF MEDICINE,MENOUFIA UNIVERSITY,SHEBEIN EL-KOM,MENOUFIA GOVERNORATE,EGYPT | ||
| 2MEDICAL BIOCHEMISTRY,FACULTY OF MEDICINE,MENOUFIA UNIVERSITY,SHEBEIN EL-KOM,MENOUFIA GOVERNORATE,EGYPT | ||
| Abstract | ||
| To evaluate the neuroprotective effect of nootropic drugs, piracetam and vincamine, on Parkinsonʹs disease (PD) in rats, forty adult male Wistar albino rats were randomized into five equal groups: control, haloperidol-induced PD group, and PD groups orally given piracetam (300 mg/kg/day), vincamine (20 mg/kg/day) or both. Four weeks later, motor performance was assessed by stepping test. Y-maze, forced swimming and olfactory preference tests were done for cognitive and behavioral evaluation. Blood samples were collected for measuring serum glucose, calcium, creatine phosphokinase (CPK) and glial cell-derived neurotrophic factor (GDNF). Thereafter, rats were sacrificed and brains were dissected. Striatal tissue of left hemisphere was isolated and homogenized for assay of dopamine, malondialdehyde (MDA), nitrite/nitrate, reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-1ß). Right hemisphere was used for histopathological examination of substantia nigra. Results: Rats of PD group showed bradykinesia, cognitive impairment, depressive-like behavior and hyposmia, reductions in serum calcium and GDNF, and in striatal dopamine, GSH, GPx and SOD, while serum glucose and CPK, and striatal MDA, nitrite/nitrate, IL-1ß and TNF-α were increased, as compared to control. Both drugs improved neurological dysfunction and biochemical parameters, as compared to PD group. The histopathological findings revealed neuro-degeneration and neuro-inflammation in PD group, that improved in treated groups. The piracetam effect was mainly anti-inflammatory, while vincamine was mainly antioxidant. Combined treatment resulted in a more potent amelioration of haloperidol-induced changes. Conclusion: Piracetam and vincamine exhibit neuroprotective activity in haloperidol-induced PD, that is more potent with their combination. | ||
| Keywords | ||
| Key words: Parkinson’s Disease; Haloperidol; Piracetam; Vincamine | ||
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