Analysis of Different Bioactive Compounds Conferring Antimicrobial Activity from Lactobacillus plantarum and Lactobacillus acidophilus with Gas Chromatography-Mass Spectrometry (GC-MS)
Dawwam, G., Saber, I., Yassin, M., Ibrahim, H. (2022). Analysis of Different Bioactive Compounds Conferring Antimicrobial Activity from Lactobacillus plantarum and Lactobacillus acidophilus with Gas Chromatography-Mass Spectrometry (GC-MS). EKB Journal Management System, 14(1), 1-10. doi: 10.21608/eajbsg.2022.213620
Ghada E. Dawwam; Israa I. Saber; M. Hisham Yassin; Hanan F. Ibrahim. "Analysis of Different Bioactive Compounds Conferring Antimicrobial Activity from Lactobacillus plantarum and Lactobacillus acidophilus with Gas Chromatography-Mass Spectrometry (GC-MS)". EKB Journal Management System, 14, 1, 2022, 1-10. doi: 10.21608/eajbsg.2022.213620
Dawwam, G., Saber, I., Yassin, M., Ibrahim, H. (2022). 'Analysis of Different Bioactive Compounds Conferring Antimicrobial Activity from Lactobacillus plantarum and Lactobacillus acidophilus with Gas Chromatography-Mass Spectrometry (GC-MS)', EKB Journal Management System, 14(1), pp. 1-10. doi: 10.21608/eajbsg.2022.213620
Dawwam, G., Saber, I., Yassin, M., Ibrahim, H. Analysis of Different Bioactive Compounds Conferring Antimicrobial Activity from Lactobacillus plantarum and Lactobacillus acidophilus with Gas Chromatography-Mass Spectrometry (GC-MS). EKB Journal Management System, 2022; 14(1): 1-10. doi: 10.21608/eajbsg.2022.213620

Analysis of Different Bioactive Compounds Conferring Antimicrobial Activity from Lactobacillus plantarum and Lactobacillus acidophilus with Gas Chromatography-Mass Spectrometry (GC-MS)

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 1, Volume 14, Issue 1, June 2022, Pages 1-10    PDF (803.38 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2022.213620
Authors
Ghada E. Dawwam1; Israa I. Saber2; M. Hisham Yassin1; Hanan F. Ibrahim3
1Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.
2Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt
3Microbiology and Immunology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
Abstract
The chemical screening tests are important tools for the detection of bioactive principles. In this study, gas chromatography−mass spectrometry (GC−MS) was successfully used to identify the biochemical constituents produced by Lactobacillus plantarum and Lactobacillus acidophilus strains. The method is based on liquid-liquid extraction followed by gas chromatography-mass spectrometry (GC-MS) analysis. The GC-MS chromatogram of the extracts of L. plantarum and L. Acidophilus revealed the presence of twenty-two and seventeen chemical compounds respectively with antimicrobial activities. Bioactive compounds as Pentadecanoic acid, Butanoic acid, Eucalyptol, Isopropyl Myristate possess, and 9-Hexadecenoic acid were detected in the extract of L. plantarum. Also, other compounds as 1- Tetradecanol, Isochiapin B, cis-Vaccenic acid, 1,2-Benzenedicarboxylic acid were detected for L. acidophilus. Furthermore, different bioactive compounds with antimicrobial activities as Oleic acid, 1-Hexadecanol, 2-methyl, 2,2- Dideutero octadecanal, 9-Octadecenoic acid were detected in both extracts of L. plantarum and L. Acidophilus. Thus, these probiotics are strong candidates as antimicrobial agents.
Keywords
Lactobacillus plantarum; Lactobacillus acidophilus; Antimicrobial; (GC; MS) analysis
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