SERO - AND MOLECULAR DIAGNOSIS FOR INCIDENCE OF Maize yellow stripe virus (MYSV) IN LOWER AND MIDDLE EGYPT
Abdel-Kader,, A., Amin, H., Aboul-Ata, A., El-Bolok, M., Hussein, A., Ammar, E. (2007). SERO - AND MOLECULAR DIAGNOSIS FOR INCIDENCE OF Maize yellow stripe virus (MYSV) IN LOWER AND MIDDLE EGYPT. EKB Journal Management System, 32(10), 8675-8684. doi: 10.21608/jppp.2007.220700
A. M. Abdel-Kader,; Hala A. Amin; A. E. Aboul-Ata; M. El-Bolok; Amal M. Hussein; E. D. Ammar. "SERO - AND MOLECULAR DIAGNOSIS FOR INCIDENCE OF Maize yellow stripe virus (MYSV) IN LOWER AND MIDDLE EGYPT". EKB Journal Management System, 32, 10, 2007, 8675-8684. doi: 10.21608/jppp.2007.220700
Abdel-Kader,, A., Amin, H., Aboul-Ata, A., El-Bolok, M., Hussein, A., Ammar, E. (2007). 'SERO - AND MOLECULAR DIAGNOSIS FOR INCIDENCE OF Maize yellow stripe virus (MYSV) IN LOWER AND MIDDLE EGYPT', EKB Journal Management System, 32(10), pp. 8675-8684. doi: 10.21608/jppp.2007.220700
Abdel-Kader,, A., Amin, H., Aboul-Ata, A., El-Bolok, M., Hussein, A., Ammar, E. SERO - AND MOLECULAR DIAGNOSIS FOR INCIDENCE OF Maize yellow stripe virus (MYSV) IN LOWER AND MIDDLE EGYPT. EKB Journal Management System, 2007; 32(10): 8675-8684. doi: 10.21608/jppp.2007.220700

SERO - AND MOLECULAR DIAGNOSIS FOR INCIDENCE OF Maize yellow stripe virus (MYSV) IN LOWER AND MIDDLE EGYPT

Journal of Plant Protection and Pathology
Article 2, Volume 32, Issue 10, October 2007, Page 8675-8684  XML PDF (677.41 K)
Document Type: Original Article
DOI: 10.21608/jppp.2007.220700
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Authors
A. M. Abdel-Kader,1; Hala A. Amin1; A. E. Aboul-Ata1; M. El-Bolok2; Amal M. Hussein3; E. D. Ammar2
1Dept. of Plant Virus and Phytoplasma Res., Plant Path. Res. Inst., ARC, P.O.Box, 12619, Giza, Egypt
2Dept. of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo, University, Giza, Egypt
3Molecular Biology Research Institute, Munofia University, Sadat City, Munofia, Egypt
Abstract
Maize Yellow Stripe Virus (MYSV) is a leafhopper-borne Tenuivirus thathas induced some epidemics on maize plants earlier in Egypt. Field samples from     maize plantations were taken during the summer and Nily growing seasons of 2004 and 2005 to estimate the occurrence of MYSV on maize in ten governorates of Middle and Lower Egypt. Disease severity and percentage of MYSV incidence were recorded. Visual examination of MYSV symptoms, serodiagnosis using DAC-ELISA, and sometimes molecular tools (IC/RT-PCR and hybridization) were used to detect the disease occurrence in maize leaf samples. Generally, MYSV occurrence was higher in Middle than in Lower Egypt. Also, it was higher in Nily than in summer plantations. However, MYSV incidence in the summer season of 2004 was higher than in Nily season of the same year. The epidemiology and transmission of this   virus by Cicadulina chinai is discussed.
Keywords
MYSV; Field Survey; Maize; DAC-ELISA; IC/RT-PCR; Hybridization
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