Molecular detection of some growth-hormone genes in chickens | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 3, Volume 100, Issue 2, June 2022, Page 193-203 PDF (1.08 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2022.101849.1163 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mohammed A. El-sayed   1; Heba A.E.M. Assi2; Saadeya S. Mekky3; Hesham A.M. Soliman4
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| 1Animal Genetic Resources Department, National Gene Bank, Agricultural Research Center, Giza, Egypt | ||||
| 2Animal Production Research Institute, Agricultural Research Center, Giza, Egypt | ||||
| 3Animal and Poultry Breeding Department, Desert Research Center, Matariya, Cairo, Egypt | ||||
| 4Animal and Poultry Breeding Department, Desert Research Center, Cairo, Egypt | ||||
| Abstract | ||||
| Due to the fact that the chicken growth hormone gene (cGH) is critical for controlling development and metabolism, there could be a link between GH polymorphisms and economic features in chickens. The objective of this study was to detect the single nucleotide polymorphisms (SNPs) in the GH and insulin-like growth factor-binding protein 2 (IGFBP2) genes, and to investigate their associations with body weight (BW). Three hundred individuals (100 birds for each commercial strains Sasso and Ross and one local breed; Baladi) were assayed. And the three primers were amplification with a total of 54 samples from three genotypes (Ross=18, Sasso=18 and Baladi=18). A total of SNPs sequenced was 138,106bp consisting of 13,265bp coding regions and 124,841bp non-coding regions. A total of 197 SNPs were identified, with 34 in coding SNPs regions and 163 in non-coding SNPs regions. The values of variation in coding regions, transitions accounted for 53% (18),26% (9) and 21% (7),while in non-coding regions were 27% (111),33% (132) and 40% (163) in Ross, Sasso and Baladi for three genes. This result is most likely due to the high, medium, and low body weights of Ross, Sasso, and Baladi breed. The higher frequency of A↔G and A↔C and lower frequency of G↔T may be related to higher body weight in the Ross strain. While, the higher frequency of A↔C, C↔T and lower G↔T may be related to lower body weight with Baladi breed. Our study confirms the analyzed three genes affecting meat production in poultry. | ||||
| Keywords | ||||
| Single Nucleotide Polymorphisms (SNPs); Growth Hormone gene (GH); Insulin-like Growth Factor Binding protein 2 (IGFBP2); Sasso; Ross strains and Baladi breed | ||||
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