NUCLEAR AREA FACTOR FOR ESTIMATION OF APOPTOSIS IN GRAVIOLA-TREATED SQUAMOUS CELL CARCINOMA CELL LINE EX-VIVO STUDY | ||||
| Ain Shams Dental Journal | ||||
| Article 11, Volume 22, Issue 2, June 2021, Page 77-83 PDF (588.69 K) | ||||
| Document Type: Original articles | ||||
| DOI: 10.21608/asdj.2021.75784.1045 | ||||
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| Authors | ||||
Ghada Mohamed Aziz   1; Houry Moustafa Baghdadi 2; Shaimaa Mustafa Masloub Ali 3
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| 1Oral pathology, Faculty Of Dentistry, Ain Shams University, Cairo, Egypt | ||||
| 2oral pathology, faculty of dentistry, ASU, cairo, egypt | ||||
| 3Oral Pathology, Faculty Of Dentistry, ain Shams University, Cairo, Egypt | ||||
| Abstract | ||||
| Aim and Objectives: This work is conducted to estimate the apoptotic effect of Graviola on tongue squamous cell carcinoma cell line by calculating the nuclear area factor (NAF) of the Graviola-treated cells. Material and Methods: Tongue squamous cell carcinoma cell line (HNO-97) was used in the current work. The drug tested in the current work was Graviola (Annona Muricata). The cytotoxicity of Graviola on cultured HNO-97 cells was evaluated by using the viability assay (MTT assay) to estimate the IC50 of Graviola at 24 hours. Cytomorphometric analysis of Gaviola-treated and untreated cells were performed to estimate the NAF using ImageJ software. Obtained data were then statistically analyzed. Results: The obtained data showed a decrease in the NAF mean values of HNO-97 cells which treated with various concentrations of Graviola at various durations as compared to untreated cells. Conclusion: The current data indicated that estimation of NAF is a sensitive technique for prediction of early apoptosis of Graviola-treated squamous cell carcinoma cell line. | ||||
| Keywords | ||||
| Graviola; Nuclear Area Factor; MTT assay | ||||
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