Comparative proteomics analyses of female Culex pipiens mosquito gut proteins after sugar and blood feeding using Nano LC-MS | ||
| Egyptian Journal of Chemistry | ||
| Volume 66, Issue 1, January 2023, Pages 335-352 PDF (1.97 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2022.126725.5629 | ||
| Authors | ||
| Rami Elansari* 1; Ahmed Saber Bream2; Wahid Hussein El-Dabae3; Shaymaa Hussein Mahmoud4 | ||
| 1Zoology and Entomology department, Faculty of Science, Al-Azhar university, Cairo, Egypt | ||
| 2Zoology and Entomology Department, Faculty of Science, Al-Azhar University, Egypt | ||
| 3Microbiology and Immunology Department, Veterinary Research Division, National Research Centre, Dokki 12622, Giza, Egypt. | ||
| 4Zoology Department, Faculty of Science, Menoufia University, Egypt | ||
| Abstract | ||
| Culex pipiens is a major vector transmitting a variety of arthropod-borne pathogens. Cx pipiens were collected then identified morphologically and by mitochondrial cytochrome oxidase I gene for accurate species confirmation. Mosquito's' gut plays an important role in infection susceptibility. A baseline proteomic dataset is needed to give insights into the physiology of blood-feeding and to understand functional proteins in mosquito behavior following a blood meal ultimately leading to establishing effective control strategies. As the changes in protein expression of Cx. pipiens gut after sugar and blood-feeding has not been investigated, a proteomic analysis of gut tissue was carried out using Nano LC/mass spectrometry (LC-MS) and offline matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-MS. There were 56 functional putative proteins in the gut of sugar-fed Cx. pipiens females and 37 proteins gut of blood-fed Cx. pipiens females with molecular weight ranging from 800.4 to 2969.4 Da and from 818.3 to 3207.6 Da for sugar and blood-fed gut females, respectively. 39 and 20 proteins were uniquely identified in sugar-fed and blood-fed, respectively, while 17 proteins were common by the two biological groups. Data are available via ProteomeXchange with identifier PXD030533. | ||
| Keywords | ||
| female; Culex pipiens; gut; blood feeding; sugar feeding; proteomics analysis | ||
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