USING RAPD-PCR TECHNIQUES IN IDENTIFICATION OF COTTON FUSARIA | ||
| Egyptian Journal of Agricultural Research | ||
| Article 2, Volume 84, Issue 2, June 2006, Pages 311-321 PDF (2.39 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2006.230295 | ||
| Author | ||
| MAGGIE E. M. HASSAN | ||
| Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt | ||
| Abstract | ||
| The development of wilt symptoms is the main criterion for the identification of such isolates A oxyspotum f. sp. vasinfectunz This biological test is time consuming and sensitive to temperature changes. Therefore, differentation between A axyspotum 1. sp. vasinfeaum isolates from the recently isolated damping-off producing isolates of A owsporum are being explored by RAPD-PCR using any of the primers d (CGTGCGGGAA)-3 or d (AAGAGCCCGT)-3 or d (AACGCGCAAC)-3 or d (CCCGTCAGCA)-3 by RAPD-PCR. The method was found sensitive, aozurate, practical and time saving in recognizing F arysporum f. sp. vasinfectum isolates, which showed a very high degree of similarity. In =bast, the damping-off causing F. oxysporum isolates tested showed different dusters with a low overall similarity. | ||
| Keywords | ||
| F. cmyspotvm f. sp. rasinfectvm; Fusarium wilt; F. oxysponim damping-off; cotton; RAPD-PCR | ||
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