Pro-active application of UV-B light could protect strawberry fruits against the postharvest gray mold
Abd El Hamid, S., Hazman, M., Zayton, M., Elwahy, A., Hamouda, S., Kabil, F. (2022). Pro-active application of UV-B light could protect strawberry fruits against the postharvest gray mold. EKB Journal Management System, 100(2), 260-270. doi: 10.21608/ejar.2022.117209.1198
Shrouk Abd El Hamid; Mohamed Hazman; Marwa Zayton; Ahmed Elwahy; Sami Hamouda; Farida Kabil. "Pro-active application of UV-B light could protect strawberry fruits against the postharvest gray mold". EKB Journal Management System, 100, 2, 2022, 260-270. doi: 10.21608/ejar.2022.117209.1198
Abd El Hamid, S., Hazman, M., Zayton, M., Elwahy, A., Hamouda, S., Kabil, F. (2022). 'Pro-active application of UV-B light could protect strawberry fruits against the postharvest gray mold', EKB Journal Management System, 100(2), pp. 260-270. doi: 10.21608/ejar.2022.117209.1198
Abd El Hamid, S., Hazman, M., Zayton, M., Elwahy, A., Hamouda, S., Kabil, F. Pro-active application of UV-B light could protect strawberry fruits against the postharvest gray mold. EKB Journal Management System, 2022; 100(2): 260-270. doi: 10.21608/ejar.2022.117209.1198

Pro-active application of UV-B light could protect strawberry fruits against the postharvest gray mold

Egyptian Journal of Agricultural Research
Article 21, Volume 100, Issue 2, June 2022, Page 260-270  XML PDF (812.27 K)
Document Type: Original Article
DOI: 10.21608/ejar.2022.117209.1198
View on SCiNiTO View on SCiNiTO
Authors
Shrouk Abd El Hamid1; Mohamed Hazman email orcid 1; Marwa Zayton2; Ahmed Elwahy3; Sami Hamouda4; Farida Kabilorcid 5
1Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt
2Plant pathology Department, Faculty of Agriculture, Cairo University, Gamma St, 12613 Giza, Egypt
3Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt
4Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt
5Vegetable Crops Department, Faculty of Agriculture, Cairo University, Giza, Egypt
Abstract
The severity of Botrytis cinerea growth could be significantly inhibited in mature strawberry fruits by priming fungal resistance in harvested berries after the proactive application of UV-B light (312 nm). Harvested fruits were irradiated by accumulative UV-B light amounts (0, 0.33, 0.65, and 1.3 mJoule/cm2) before being artificially infected with B. cinerea. PCR assay could estimate a reduction ratio of 50 % in fungal DNA in fruits proactively exposed to UV-B light (1.3 mJoule/cm2). These fruits showed a minimum fungal severity level compared to non-UV-B treated fruits (0 mJoule/cm2), which were harshly rotted with velvety gray mold growth. To reveal a side of possible molecular mechanisms behind UV-B induced immunity in ripe berries against B. cinerea, the expression profiles of some resistance-related genes were quantified using real-time PCR on day 1 and day 2 after infection. UV-B significantly sharpens the expression of strawberry defense gene FaBG2-1 which encodes the fungal cell wall degrading enzyme β-1-3-glucanase. Furthermore, jasmonic acid (JA) biosynthesis key gene FaAOS was strongly up-regulated after exposure to UV-B in infected or non-infected fruits on day 2. Equally important, the expression of FaNES1, volatile terpenoids linalool/nerolidol synthase gene, was moderately elevated. ABA signaling gene FaPYR1 was found to be more responsive on day 2 after UV-B irradiation. Collectively, we hypothesize that UV-B proactive irradiation can encourage the enhancement of the molecular immune system in mature strawberry fruits against possible B. cinerea infection postharvest. Further studies are needed to reveal UV-B potential as a sustainable priming agent against plant diseases.
Keywords
Strawberry; Botrytis cinerea; UV-B; gene expression
Statistics
Article View: 544
PDF Download: 667