GC-MS and HPLC analyses of phytochemical compounds from Withania somnifera L. leaves extract | ||||
| Alexandria Journal of Agricultural Sciences | ||||
| Article 1, Volume 67, Issue 1, March 2022, Page 10-17 PDF (509.75 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/alexja.2022.131511.1016 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mervat EL-Hefny1; Mamoun S.M. Abd El-Kareem2; Mohamed Z.M. Salem   3
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| 1Department of Floriculture, Ornamental Horticulture and Garden Design, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt | ||||
| 2Atomic and Molecular Physics Unit, Experimental Nuclear Physics Department, Nuclear Research Centre, Egyptian Atomic Energy Authority, Inshas, Cairo, Egypt | ||||
| 3Forestry and Wood Technology Department, Faculty of Agriculture (El–Shatby), Alexandria University, Egypt | ||||
| Abstract | ||||
| In the present work, the shrub W. somnifera was used as a source for the extraction of bioactive molecules. Leaves were extracted by ethanol and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC). By GC-MS analysis, the highest identified compounds were 2,6-dimethyl-N-(2-methyl-α-phenylbenzyl)aniline, 4-Aminoheptane, n-undecanophenone, 2-cyclohexyl-4-hydroxymethyl-octahydrobenzo[e][1,2] oxazine-3-carbonitrile, and octadecanoic acid 3-octadecyloxypropyl ester, with percentages of 19.43%, 7.51%, 4.47%, 4.34%, and 3.89%, respectively. HPLC analysis identified quercetin and 7-hydroxyflavone with concentrations of 12.36 and 15.14 mg/mL, respectively, as two flavonoid compounds. This work showed that W. somnifera leaf extract owns several bioactive compounds that could be useful and have different applications, such as antimicrobial activities. | ||||
| Keywords | ||||
| Withania somnifera leaves; HPLC; GC-MS; quercetin; 7-hydroxyflavone | ||||
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