Hepatoprotective impact of Boldo (Peumus boldus) extract against azoxystrobin induced DNA damage, gene expression modulation, biochemical and histopathological alterations mediated-ROS generation in male rats
Refaie, A., Shalby, A., Booles, H., Kassem, S., Eshak, M., Farrag, A., Khalil, W. (2022). Hepatoprotective impact of Boldo (Peumus boldus) extract against azoxystrobin induced DNA damage, gene expression modulation, biochemical and histopathological alterations mediated-ROS generation in male rats. EKB Journal Management System, 65(8), 687-698. doi: 10.21608/ejchem.2022.120306.5401
Aml Refaie; Aziza Bakr Shalby; Hoda F. Booles; Salwa M. Kassem; Mariam G. Eshak; Abde Razik H. Farrag; Wagdy K. B. Khalil. "Hepatoprotective impact of Boldo (Peumus boldus) extract against azoxystrobin induced DNA damage, gene expression modulation, biochemical and histopathological alterations mediated-ROS generation in male rats". EKB Journal Management System, 65, 8, 2022, 687-698. doi: 10.21608/ejchem.2022.120306.5401
Refaie, A., Shalby, A., Booles, H., Kassem, S., Eshak, M., Farrag, A., Khalil, W. (2022). 'Hepatoprotective impact of Boldo (Peumus boldus) extract against azoxystrobin induced DNA damage, gene expression modulation, biochemical and histopathological alterations mediated-ROS generation in male rats', EKB Journal Management System, 65(8), pp. 687-698. doi: 10.21608/ejchem.2022.120306.5401
Refaie, A., Shalby, A., Booles, H., Kassem, S., Eshak, M., Farrag, A., Khalil, W. Hepatoprotective impact of Boldo (Peumus boldus) extract against azoxystrobin induced DNA damage, gene expression modulation, biochemical and histopathological alterations mediated-ROS generation in male rats. EKB Journal Management System, 2022; 65(8): 687-698. doi: 10.21608/ejchem.2022.120306.5401

Hepatoprotective impact of Boldo (Peumus boldus) extract against azoxystrobin induced DNA damage, gene expression modulation, biochemical and histopathological alterations mediated-ROS generation in male rats

Egyptian Journal of Chemistry
Article 61, Volume 65, Issue 8, August 2022, Page 687-698  XML PDF (647.47 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.120306.5401
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Authors
Aml Refaieorcid 1; Aziza Bakr Shalby email orcid 2; Hoda F. Booles3; Salwa M. Kassemorcid 4; Mariam G. Eshakorcid 3; Abde Razik H. Farragorcid 5; Wagdy K. B. Khalilorcid 6
1Pesticide chemistery
22Department of Hormones, Medicine and clinical studies research institute, National Research Centre (NRC), Cairo, Egypt
3Department of Cell Biology, Biotechnology research institute, National Research Centre, Cairo, Egypt
4Department of Cell Biology, Biotechnology Research Institute, National Research Centre, 33-Bohouth St., P.O. 12622 Dokki, Cairo, Egypt
5Pathology Department, Medical Research and Clinical Studies Institute, National Research Centre
6Cell Biology Department, National Research Centre, 33 Bohouth St., 12622 Dokki, Giza, Egypt
Abstract
Peumus boldus Molina, ordinarily mentioned to as ‘boldo’, is expended in folk medicine to cure gastrointestinal and hepatic diseases. This plant is wealthy in several antioxidant compounds. The current research evaluated the ameliorative effect of boldo leaves extract on lipid peroxidation, liver antioxidant enzymes, ROS generation, DNA damage, gene expression, and histopathological changes induced by Amistar fungicide in male rats. The rats were allocated in four groups treated with boldo extract (50 mg/Kg b.w) and/or with Amistar (1/20 of LD50). As likely, the treatment with fungicide significantly (P<0.05) decreased the antioxidant enzymes activities, while increasing the liver biomarker parameters, lipid peroxidation, ROS generation, DNA damage, and hepatic tissue lesions as well as altered the expression of stress and antioxidant related genes in comparison with control rats. Pretreatment with boldo extract significantly improved the negative effects induced by Amistar on the liver cells. The research results indicate that the boldo extract acts as a defender against the oxidative hepatic injury produced by fungicide. The defensive capability of boldo extract could be owing to the existence of natural antioxidants and anti-inflammatory constituents’ likes gallic acid, ellagic acid, querectin, daidzein, ferulic acid, and rutin.
Keywords
Peumus boldus; Amistar; Lipoperoxidation; Antioxidants; ROS, DNA damage
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