Molecular Characterization of Some Egyptian cotton Varieties | ||
| Alexandria Science Exchange Journal | ||
| Article 6, Volume 38, January-March - Serial Number 1, March 2017, Pages 44-52 PDF (1.19 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/asejaiqjsae.2017.2319 | ||
| Authors | ||
| Issraa Saif1; Seehy M.A1; Sanaa Riad2; Mahmoud Elbagoury2 | ||
| 1Department of Genetics, Faculty of Agriculture, Alexandria University. | ||
| 2Cotton Arbitration and Testing General Organization (CATGO) | ||
| Abstract | ||
| Cottonplant belongs to the genus Gossypium which is considered as the first important economic crop in Egypt. The present study aims to characterize five Egyptian cotton varieties (Giza(G) 92;Giza(G) 93; Giza(G)86; Giza(G)87and Giza(G)88) at biochemical and molecular level. Using ISSR; SSR and RAPD-PCR analyses, a total of 159 polymorphic fragments were recorded with similarity of 40; 23.5 and 38%, respectively, for the five varieties. The Phylogenetic relationships of the five Egyptian cotton varieties based on DNA molecular data (RAPD + ISSR + SSR) indicated two groups with 56% similarity; the first group had (G88); However, G93 and G86 with homology percentage of 81%, and both of them in relation to G87 variety with approximately 65.5% similarity. | ||
| Keywords | ||
| Cotton; molecular markers; ISSR; SSR; RAPD-PCR | ||
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