CRYPTOSPORIDIOSIS: MOLECULAR ANALYSIS, RISK FACTORS AND SEASONAL ABUNDANCE IN IMMUNOCOMPETENT AND IMMUNOCOMPROMISED PATIENTS, KAFRELSHEIKH UNIVERSITY HOSPITALS | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 14, Volume 52, Issue 1, May 2022, Pages 117-122 PDF (802.02 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2022.235822 | ||
| Authors | ||
| MARWA M.I. GHALLAB1; MOUSA A. M. ISMAIL2; TAMER HAYDARA3; SALWA M. MORSY2 | ||
| 1Departments of Medical Parasitology, Faculty of Medicine, Kafrelsheikh University | ||
| 2Departments of Medical Parasitology, Faculty of Medicine, Cairo University | ||
| 3Departments of Internal Medicine, Faculty of Medicine, Kafrelsheikh University | ||
| Abstract | ||
| This study detected of the molecular Cryptosporidium species in immunocompetent and immunocompromised adults with diarrhea attended Kafrelsheikh University Hospitals and to assess risk factors. Stool samples from 200 immunocompetent & 200 immunocompromised were examined using acid fast (AF) stain and PCR/restriction fragment length polymorphism (RFLP) targeting small subunit (SSU) rRNA gene. Cryptosporidium PCR amplified was 15(7.5%) in immunocompetent and 23 (11.5%) immunocompromised, with significance difference as to age, presence of mucous, abdominal pain (P<0.05). Cryptosporidium was more common in rural areas in autumn and winter seasons. Two genotypes were C. hominis and C. parvum, but C. hominis was 60% in immunocompetent and 82.6% in immunocompromised. | ||
| Keywords | ||
| Patients; immunocompetent; immunocompromized; Cryptosporidiosis; SSU gene; RFLP | ||
|
Statistics Article View: 248 PDF Download: 284 |
||
