IMMUNOCHROMATOGRAPHY VERSUS MICROSCOPY FOR DIAGNOSIS OF ENTAMOEBIA HISTOLYTICA/DISPAR INFECTION IN S OHAG UNIVERSITY HOSPITALS, EGYPT | ||||
Journal of the Egyptian Society of Parasitology | ||||
Article 17, Volume 52, Issue 1, May 2022, Page 139-144 PDF (955.75 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jesp.2022.235838 | ||||
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Authors | ||||
Asmaa kamal Abd ellah1; AMAL MOSTAFA AHMED2; KHOULOOD ZAKARIA HASHEM2; ASMAA KAMAL ABD ELLAH1 | ||||
1Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt | ||||
2Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt. | ||||
Abstract | ||||
Amoebiasis caused by E. histolytica protozoon, is a major public health worldwide. Delay in diagnosis and treatment may lead to fatality. This study evaluated the efficacy of immunochromatographic test (ICT) using RIDA®QUICK Entamoeba test compared with traditional microscopic examination for E. histolytica /dispar infection. A cross-sectional study was conducted on 100 patients suffering from dysentery or diarrhea with an age range of 3-62 years, attended Endemic Diseases Hospital, Sohag University Hospitals. Stool samples were examined by direct wet smears, formalin ethyl acetate concentration methods, stained with Wiegert’s iron hematoxylin and Modified Ziehl-Neelsen stains. Microscopic examination showed 43/100 (43%) patients having E. histolytica/dispar. Copro-antigen detection in stool sample was done using Rida ®Quick Entamoeba test. The sensitivity, specificity, PPV & NPV of Rida®Quick Enta-moeba tests were 97.67%, 96.49%, 95.45% & 98.21% respectively with an accuracy of 97.7%, and without cross-reactivity with some endemic intestinal parasites. The allover infection rate was significantly associated with residence (P < 0.036) and family size (P < 0.03), but neither associated with sex (P < 0.471) nor age (P < 0.344). Rida® Quick Entamoeba test proved to be simple, rapid, convenient, sensitive and specific assay to diagnose E. histolytica /dispar infection for epidemiology. | ||||
Keywords | ||||
Rida®Quick Entamoeba test; ICT; Microscopy; Entamoebia histolytica/dispar | ||||
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