IMMUNOCHROMATOGRAPHY VERSUS MICROSCOPY FOR DIAGNOSIS OF ENTAMOEBIA HISTOLYTICA/DISPAR INFECTION IN S OHAG UNIVERSITY HOSPITALS, EGYPT
Abd ellah, A., AHMED, A., HASHEM, K., ABD ELLAH, A. (2022). IMMUNOCHROMATOGRAPHY VERSUS MICROSCOPY FOR DIAGNOSIS OF ENTAMOEBIA HISTOLYTICA/DISPAR INFECTION IN S OHAG UNIVERSITY HOSPITALS, EGYPT. EKB Journal Management System, 52(1), 139-144. doi: 10.21608/jesp.2022.235838
Asmaa kamal Abd ellah; AMAL MOSTAFA AHMED; KHOULOOD ZAKARIA HASHEM; ASMAA KAMAL ABD ELLAH. "IMMUNOCHROMATOGRAPHY VERSUS MICROSCOPY FOR DIAGNOSIS OF ENTAMOEBIA HISTOLYTICA/DISPAR INFECTION IN S OHAG UNIVERSITY HOSPITALS, EGYPT". EKB Journal Management System, 52, 1, 2022, 139-144. doi: 10.21608/jesp.2022.235838
Abd ellah, A., AHMED, A., HASHEM, K., ABD ELLAH, A. (2022). 'IMMUNOCHROMATOGRAPHY VERSUS MICROSCOPY FOR DIAGNOSIS OF ENTAMOEBIA HISTOLYTICA/DISPAR INFECTION IN S OHAG UNIVERSITY HOSPITALS, EGYPT', EKB Journal Management System, 52(1), pp. 139-144. doi: 10.21608/jesp.2022.235838
Abd ellah, A., AHMED, A., HASHEM, K., ABD ELLAH, A. IMMUNOCHROMATOGRAPHY VERSUS MICROSCOPY FOR DIAGNOSIS OF ENTAMOEBIA HISTOLYTICA/DISPAR INFECTION IN S OHAG UNIVERSITY HOSPITALS, EGYPT. EKB Journal Management System, 2022; 52(1): 139-144. doi: 10.21608/jesp.2022.235838

IMMUNOCHROMATOGRAPHY VERSUS MICROSCOPY FOR DIAGNOSIS OF ENTAMOEBIA HISTOLYTICA/DISPAR INFECTION IN S OHAG UNIVERSITY HOSPITALS, EGYPT

Journal of the Egyptian Society of Parasitology
Article 17, Volume 52, Issue 1, May 2022, Pages 139-144    PDF (955.75 K)
Document Type: Original Article
DOI: 10.21608/jesp.2022.235838
Authors
Asmaa kamal Abd ellah1; AMAL MOSTAFA AHMED2; KHOULOOD ZAKARIA HASHEM2; ASMAA KAMAL ABD ELLAH1
1Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt
2Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt.
Abstract
Amoebiasis caused by E. histolytica protozoon, is a major public health worldwide. Delay in
diagnosis and treatment may lead to fatality. This study evaluated the efficacy of immunochromatographic
test (ICT) using RIDA®QUICK Entamoeba test compared with traditional microscopic
examination for E. histolytica /dispar infection. A cross-sectional study was conducted on
100 patients suffering from dysentery or diarrhea with an age range of 3-62 years, attended Endemic
Diseases Hospital, Sohag University Hospitals. Stool samples were examined by direct
wet smears, formalin ethyl acetate concentration methods, stained with Wiegert’s iron hematoxylin
and Modified Ziehl-Neelsen stains. Microscopic examination showed 43/100 (43%) patients
having E. histolytica/dispar. Copro-antigen detection in stool sample was done using Rida
®Quick Entamoeba test. The sensitivity, specificity, PPV & NPV of Rida®Quick Enta-moeba
tests were 97.67%, 96.49%, 95.45% & 98.21% respectively with an accuracy of 97.7%, and
without cross-reactivity with some endemic intestinal parasites. The allover infection rate was
significantly associated with residence (P < 0.036) and family size (P < 0.03), but neither associated
with sex (P < 0.471) nor age (P < 0.344). Rida® Quick Entamoeba test proved to be simple,
rapid, convenient, sensitive and specific assay to diagnose E. histolytica /dispar infection for epidemiology.
Keywords
Rida®Quick Entamoeba test; ICT; Microscopy; Entamoebia histolytica/dispar
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