EXPLORATION OF COMPONENTS CONTRIBUTING TO POTENT CYTOTOXICITY OF GARDENIA THUNBERGIA L. F. AGAINST HUMAN LEUKEMIA AND HEPATOMA | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Article 13, Volume 45, Issue 1, June 2022, Pages 153-162 PDF (902.96 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2022.239374 | ||
| Authors | ||
| Shaymaa M. Mohamed1; Samir Ross2, 3; Nesma M. Mohamed* 4 | ||
| 1Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71515, Egypt | ||
| 2National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, Mississippi 38677, USA | ||
| 3Department of BioMolecular Sciences, Division of Pharmacognosy, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA | ||
| 4Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71515, Egypt. | ||
| Abstract | ||
| Promising cytotoxic effects of several Gardenia species (Rubiaceae) have been established by many studies. The current study evaluated MTT-based cytotoxic activities of the crude extract from Gardenia thunbergia L. f. aerial parts and four fractions thereof, including n-hexane, dichloromethane (DCM), ethyl acetate, and aqueous, against human leukemia (HL-60) and hepatoma (HepG2) cell lines, as well as the normal (WI38) cell line. Both non-polar fractions, n-hexane and dichloromethane, showed tumor-selective toxicities against both tested cancerous cell lines. These results sparked our interest in chemically characterising these bioactive fractions to reveal their cytotoxic components. The composition of n-hexane-soluble fraction was investigated via GC-MS analysis, while column chromatographic separation was used to isolate the components of DCM-soluble fraction. These isolated phytochemicals were identified via spectroscopic analyses. Besides, the chemotaxonomic value of the detected phytochemicals and their reported cytotoxic profiles were discussed. | ||
| Keywords | ||
| Gardenia thunbergia; Rubiaceae; Cytotoxicity; Human leukemia; HepG2 cells; Chemotaxonomy | ||
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