Sirtuin 1 Gene Expression in Non-alcoholic Fatty Liver Disease and Its Correlation with Biochemical and Clinical Features
sulayman, S., Hamed, E., Ali, S., Ibrahim, T. (2022). Sirtuin 1 Gene Expression in Non-alcoholic Fatty Liver Disease and Its Correlation with Biochemical and Clinical Features. EKB Journal Management System, 88(1), 2606-2612. doi: 10.21608/ejhm.2022.240881
shadi sulayman; Emad Fawzi Hamed; Samia Hussein Ali; Tarek M. H. Ibrahim. "Sirtuin 1 Gene Expression in Non-alcoholic Fatty Liver Disease and Its Correlation with Biochemical and Clinical Features". EKB Journal Management System, 88, 1, 2022, 2606-2612. doi: 10.21608/ejhm.2022.240881
sulayman, S., Hamed, E., Ali, S., Ibrahim, T. (2022). 'Sirtuin 1 Gene Expression in Non-alcoholic Fatty Liver Disease and Its Correlation with Biochemical and Clinical Features', EKB Journal Management System, 88(1), pp. 2606-2612. doi: 10.21608/ejhm.2022.240881
sulayman, S., Hamed, E., Ali, S., Ibrahim, T. Sirtuin 1 Gene Expression in Non-alcoholic Fatty Liver Disease and Its Correlation with Biochemical and Clinical Features. EKB Journal Management System, 2022; 88(1): 2606-2612. doi: 10.21608/ejhm.2022.240881

Sirtuin 1 Gene Expression in Non-alcoholic Fatty Liver Disease and Its Correlation with Biochemical and Clinical Features

The Egyptian Journal of Hospital Medicine
Article 52, Volume 88, Issue 1, July 2022, Page 2606-2612  XML PDF (602.63 K)
DOI: 10.21608/ejhm.2022.240881
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Authors
shadi sulayman* 1; Emad Fawzi Hamed1; Samia Hussein Ali2; Tarek M. H. Ibrahim1
1Departments of 1Internal Medicine
22Medical Biochemistry, Faculty of Medicine, Zagazig University, Egypt
Abstract
Background: Silent information regulator 1 [sirt1] is a protein that is widely expressed and has a crucial function in the prevention of oxidative stress, which is implicated in the development of a number of chronic disorders, such as fatty liver disease.
Objective: The aim of the current work was to investigate SIRT1 gene expression in non-alcoholic fatty liver disease and it is emerging role in pathogenesis of non-alcoholic fatty liver disease. Subjects and Methods: This case-control study included a total of twenty cases of Non-Alcoholic Fatty Liver Disease; NAFLD (Group A) and ten individuals as a control (Group B), attending at Departments of Internal Medicine and Medical Biochemistry, Zagazig University Hospitals. They were assessed clinically by lab investigations and quantitative real time polymerase chain reaction (RT-PCR) to assess Sirt1 gene fold expression.
Results: There was a significant lowering of sirt1 gene expression p=0.0001 for patient with NAFLD compared to healthy controls. There was significant association between SIRT1 and BMI, as well as hyperlipidemia in NAFLD compared to healthy group. We found a significant association between SIRT1 and NAFLD patients with mild fibrosis and those without fibrosis. Cut off value ≤0.61 had 80.0% sensitivity and 80.0% specificity, positive predictive value 57.0%, negative predictive value 92.0% and accuracy was 80.0%.
Conclusion: It could be concluded that the significant correlation between SIRT1 gene expression and non-alcoholic fatty liver disease helps to differentiate patients with nonalcoholic fatty liver diseases and healthy ones.
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