The Potential Anti-ulcerogenic Effects of Baicalein and/or Empagliflozin in Induced Gastric Ulcer in Rats: Modulating HO-1/SIRT1 / HMGB1 signaling Pathway | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 3, Volume 42, Issue 3, July 2022, Pages 246-264 PDF (954.42 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2022.118340.1116 | ||
| Authors | ||
| Elham Nasif* 1; Rania Shalaby2; Sarah Ragab Abd El -Khalik3; Rash Abd Ellatif4 | ||
| 1Medical Physiology departement, Faculty of Medicine, Tanta University, Tanta, Egypt | ||
| 2Pharmacology Department, Faculty of Medicine, Tanta University, Tanta, Egypt. | ||
| 3Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta, Egypt. | ||
| 4Anatomy and Embryology Department, Faculty of Medicine, Tanta University, Tanta, Egypt. | ||
| Abstract | ||
| Background: Gastric ulcer is one of the most ubiquitous gastrointestinal tract disorders, causing high morbidity. The goal of this study was to explore at the mechanistic effects of Baicalein (BA) and/or Empagliflozin (EMP) treatment on gastric ulcer caused by indomethacin and prednisolone, with an emphasis on the role of the HO-1/SIRT1 / HMGB1 signaling pathway. Material and methods: Fifty adult albino rats were allocated into 5 equal groups: control, ulcer (Prednisolone 10mg/kg for 6 days followed by indomethacin as single oral dose 30 mg/kg), BA (30 mg/kg), EMP (10 mg/kg), and BA + EMP groups. The volume of gastric juice, pH, free and total acidity was estimated. The gene expression of HO-1 and SIRT1 in gastric tissues was assessed by qRT -PCR. Biochemical analysis of gastric tissues homogenates including HMGB-1, PGE2 & nitrites levels was performed. Assay of inflammatory markers and redox status were detected. Additionally, histological, scanning electron microscopic and immunohistochemical analyses were determined. Results: After 4 weeks of treatment, there was remarkable improvement of the histological architecture of rat gastric tissues. Upregulation of HO-1 and SIRT1 gene expression, as well as a decrease in HMGB1 level, resulted in improved inflammatory and oxidative stress biomarkers. Furthermore, immunohistochemical analysis revealed increase in Bcl-2 expression and decreased expression of Bax in the treated groups. Conclusion: Concurrent usage of BA & EMP against gastric ulcer in rats could be related to the interaction of their anti-oxidant, anti-inflammatory, and anti-apoptotic activities via modulation of HO-1/SIRT1 / HMGB1 signaling pathway. | ||
| Keywords | ||
| Gastric ulcer; Baicalein; Empagliflozin | ||
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