Association between Interleukin-6 Promoter Polymorphism (-174 G/C), Serum Interleukin-6 Levels and severity of sepsis
Mostafa, M., ELshourbagy, E., Shahat, A. (2022). Association between Interleukin-6 Promoter Polymorphism (-174 G/C), Serum Interleukin-6 Levels and severity of sepsis. EKB Journal Management System, 31(3), 77-81. doi: 10.21608/ejmm.2022.247222
Mysa Saeed Mostafa; Eman wagih ELshourbagy; Amany Shahat. "Association between Interleukin-6 Promoter Polymorphism (-174 G/C), Serum Interleukin-6 Levels and severity of sepsis". EKB Journal Management System, 31, 3, 2022, 77-81. doi: 10.21608/ejmm.2022.247222
Mostafa, M., ELshourbagy, E., Shahat, A. (2022). 'Association between Interleukin-6 Promoter Polymorphism (-174 G/C), Serum Interleukin-6 Levels and severity of sepsis', EKB Journal Management System, 31(3), pp. 77-81. doi: 10.21608/ejmm.2022.247222
Mostafa, M., ELshourbagy, E., Shahat, A. Association between Interleukin-6 Promoter Polymorphism (-174 G/C), Serum Interleukin-6 Levels and severity of sepsis. EKB Journal Management System, 2022; 31(3): 77-81. doi: 10.21608/ejmm.2022.247222

Association between Interleukin-6 Promoter Polymorphism (-174 G/C), Serum Interleukin-6 Levels and severity of sepsis

Egyptian Journal of Medical Microbiology
Volume 31, Issue 3, July 2022, Page 77-81  XML PDF (325.93 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2022.247222
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Authors
Mysa Saeed Mostafa email 1; Eman wagih ELshourbagy2; Amany Shahat3
1Microbiology and immunology department faculty of medicine benha university
2rehumatology and rehabilitation benha faculty of medicine benha university
3Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University
Abstract
Background: Sepsis is a complicated clinical syndrome resulting from inflammatory reactions to bacteria or their products.  IL-6 is a potent inflammatory cytokine produced by many cells, such as leucocytes, monocytes, endothelial cells, and fibroblasts.  IL-6 plays an important role in the immune response and inflammation reactions   regulation. Objective: the present study aimed to detect if IL-6 serum level and polymorphism correlate with severity of sepsis.  Methodology: The study includes 70 subjects, 50 patients (Case) were chosen with at least 2 SIRS criteria and 20 healthy who were age matched.  The patients were categorized into systemic inflammatory response syndrome (SIRS), sepsis, severe sepsis, septic shock and multi-organ dysfunction syndrome (MODS) according to ACCP/SCCM. Bactec Culture on automated blood culture system (Bact/ALERT 3D) was used as a  gold standard method to prove sepsis .  Serum IL-6 was measured by Enzyme linked immunosorbent assay (ELISA). Genotyping of the IL-6–174 G/C promoter polymorphism was performed using restriction fragment length polymorphism (RFLP) analysis. Results: Our study reported a statistically high significant difference of   the IL-6 values between the case and control groups where p<0.001 and there was increasing values of serum IL-6 parallel with the developing  stages of sepsis. Also, the IL-6 values in the bacteriological culture positive and negative groups showed  differences  of statistically significant ratios (p<0.05 ).  Conclusion: Our findings reported a significant association between IL-6 promoter polymorphism (-174 G/C), levels of IL6  and severity of sepsis . 
Keywords
IL6; sepsis; ELIZA; polymorphism
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