Association between Interleukin-6 Promoter Polymorphism (-174 G/C), Serum Interleukin-6 Levels and severity of sepsis | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 31, Issue 3, July 2022, Pages 77-81 PDF (325.93 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2022.247222 | ||
| Authors | ||
| Mysa Saeed Mostafa* 1; Eman wagih ELshourbagy2; Amany Shahat3 | ||
| 1Microbiology and immunology department faculty of medicine benha university | ||
| 2rehumatology and rehabilitation benha faculty of medicine benha university | ||
| 3Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University | ||
| Abstract | ||
| Background: Sepsis is a complicated clinical syndrome resulting from inflammatory reactions to bacteria or their products. IL-6 is a potent inflammatory cytokine produced by many cells, such as leucocytes, monocytes, endothelial cells, and fibroblasts. IL-6 plays an important role in the immune response and inflammation reactions regulation. Objective: the present study aimed to detect if IL-6 serum level and polymorphism correlate with severity of sepsis. Methodology: The study includes 70 subjects, 50 patients (Case) were chosen with at least 2 SIRS criteria and 20 healthy who were age matched. The patients were categorized into systemic inflammatory response syndrome (SIRS), sepsis, severe sepsis, septic shock and multi-organ dysfunction syndrome (MODS) according to ACCP/SCCM. Bactec Culture on automated blood culture system (Bact/ALERT 3D) was used as a gold standard method to prove sepsis . Serum IL-6 was measured by Enzyme linked immunosorbent assay (ELISA). Genotyping of the IL-6–174 G/C promoter polymorphism was performed using restriction fragment length polymorphism (RFLP) analysis. Results: Our study reported a statistically high significant difference of the IL-6 values between the case and control groups where p<0.001 and there was increasing values of serum IL-6 parallel with the developing stages of sepsis. Also, the IL-6 values in the bacteriological culture positive and negative groups showed differences of statistically significant ratios (p<0.05 ). Conclusion: Our findings reported a significant association between IL-6 promoter polymorphism (-174 G/C), levels of IL6 and severity of sepsis . | ||
| Keywords | ||
| IL6; sepsis; ELIZA; polymorphism | ||
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