Approach for Quantification of Nanogram Amounts of Hepatitis-C Drugs, Sofosbuvir, and Daclatasvir based on Cerium (IV) Oxidation
Mohamed, S., Issa, Y., Al Ansary, A., Mahmoud, A. (2023). Approach for Quantification of Nanogram Amounts of Hepatitis-C Drugs, Sofosbuvir, and Daclatasvir based on Cerium (IV) Oxidation. EKB Journal Management System, 66(3), 449-457. doi: 10.21608/ejchem.2022.142020.6214
Sabrein Mohamed; yousry Issa; Aida L Al Ansary; Ahmed Mahmoud. "Approach for Quantification of Nanogram Amounts of Hepatitis-C Drugs, Sofosbuvir, and Daclatasvir based on Cerium (IV) Oxidation". EKB Journal Management System, 66, 3, 2023, 449-457. doi: 10.21608/ejchem.2022.142020.6214
Mohamed, S., Issa, Y., Al Ansary, A., Mahmoud, A. (2023). 'Approach for Quantification of Nanogram Amounts of Hepatitis-C Drugs, Sofosbuvir, and Daclatasvir based on Cerium (IV) Oxidation', EKB Journal Management System, 66(3), pp. 449-457. doi: 10.21608/ejchem.2022.142020.6214
Mohamed, S., Issa, Y., Al Ansary, A., Mahmoud, A. Approach for Quantification of Nanogram Amounts of Hepatitis-C Drugs, Sofosbuvir, and Daclatasvir based on Cerium (IV) Oxidation. EKB Journal Management System, 2023; 66(3): 449-457. doi: 10.21608/ejchem.2022.142020.6214

Approach for Quantification of Nanogram Amounts of Hepatitis-C Drugs, Sofosbuvir, and Daclatasvir based on Cerium (IV) Oxidation

Egyptian Journal of Chemistry
Volume 66, Issue 3, March 2023, Pages 449-457    PDF (529.8 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.142020.6214
Authors
Sabrein Mohamed* 1; yousry Issa2; Aida L Al Ansary3; Ahmed Mahmoud4
11-Department of chemistry, Faculty of science, Cairo University, Giza, Egypt 2-Chemistry Department, College of Science, Jouf University, P.O. Box 2014, Sakaka, Saudi Arabia
2Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt
3Dept. of chemistry, Faculty of Science, Cairo University
4National Center for Environmental and Clinical Toxicology, Cairo University, Giza, Egypt
Abstract
Sofosbuvir (SOFO) and Daclatasvir (DK) are known as hepatitis-C drugs. Through oxidation of these drugs in a sulfuric acid medium by an excess of Cerium (IV) and the subsequent determination of unreacted Cerium (IV), this research developed spectrophotometric methods for assessing the effectiveness of these hepatitis-C drugs. As an indicator of excessive Cerium (IV), we measured the decrease in the color of four chromotropic acid azo dyes, Chromotrope 2B (method A), Arsenazo I (method B), Spadns (method C), and Sulfonazo III (method D) at 510, 499, 505, and 570 nm, respectively. According to Beer's plots, there was a significant relationship between 0.1-2.1 and 0.1-5.1 µg mL-1 with molar absorptivity values of 1.7x105, 2.7x105, 1.4x105, and 1.7x105 for Sofosbuvir and 1.2x105, 7.0x104, 6.2x104, and 9.0x104 for Daclatasvir utilizing methods Chromotrope 2B (method A), Arsenazo I (method B), Spadns (method C), and Sulfonazo III (method D), respectively. To assess the efficacy of the suggested process, pure and pharmaceutical solutions containing Sofosbuvir and Daclatasvir were tested.
Keywords
Chromotropic azo dyes. Cerium (IV). Sofosbuvir. Daclatasvir
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