EVALUATION OF THE EFFICACY OF PROPOLIS AS STORAGE MEDIA FOR AVULSED TEETH | ||
| Egyptian Dental Journal | ||
| Article 12, Volume 68, Issue 3 - Serial Number 1, July 2022, Pages 2115-2121 PDF (425.53 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/edj.2022.138017.2105 | ||
| Authors | ||
| Nada Abdelbaseer* 1; Nagwa Khattab2; Mona Mahmoud3 | ||
| 1Dentist at Ministry of Health, B.D.S, Faculty of Dentistry, Minia University | ||
| 2Professor of Pediatric Dentistry and Dental Public Health, Faculty of Dentistry, Ain Shams University. | ||
| 3Lecturer of Pediatric and Community Dentistry, Faculty of Dentistry, Minia University. | ||
| Abstract | ||
| ABSTRACT Background: The best treatment for tooth avulsion is immediate replantation as it maintains the periodontal ligament (PDL) cell viability. If not possible, the tooth must be kept in a suitable storage medium before seeking dental treatment. Aim of the study: Evaluation of the efficacy of propolis as storage media in maintaining periodontal ligament cell viability for different time periods, in comparison to milk. Materials and Methods: Immortalized human skin fibroblasts were cultured and stored in the following media: Dulbecco’s modified Eagle’s medium (DMEM), pasteurized low fat content milk and two different concentrations of propolis (10%, and 20%) that were prepared by diluting stock solution of propolis with either Phosphate buffer saline (PBS) or DMEM or milk for time points of 1, 3, 6 and 12 hours. Cell viability was determined using the SRB assay. Results: Propolis had a significantly higher percentage of viable cells survived in propolis 10%, 20% storage media in comparison to other storage media: milk, DMEM and PBS through different time periods. Conclusion: Propolis could be a good naturally available storage media for avulsed teeth. | ||
| Keywords | ||
| Avulsion; Cell viability; Storage media; Propolis; Milk | ||
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