Phytochemical Profile of Phoenix rupicola T. Anderson Seeds and In Vitro Evaluation of their Estrogenic Activity using MCF-7 Cell Lines
AbouZeid, E., Hussein, R., Afifi, A., Salama, A., Youssef, F., El-Ahmady, S., Ammar, N. (2022). Phytochemical Profile of Phoenix rupicola T. Anderson Seeds and In Vitro Evaluation of their Estrogenic Activity using MCF-7 Cell Lines. EKB Journal Management System, 65(132), 1457-1464. doi: 10.21608/ejchem.2022.129433.5731
Enaam AbouZeid; Rehab Hussein; Ahmed Afifi; Abeer Salama; Fadia Youssef; Sherweit El-Ahmady; Nagwa Ammar. "Phytochemical Profile of Phoenix rupicola T. Anderson Seeds and In Vitro Evaluation of their Estrogenic Activity using MCF-7 Cell Lines". EKB Journal Management System, 65, 132, 2022, 1457-1464. doi: 10.21608/ejchem.2022.129433.5731
AbouZeid, E., Hussein, R., Afifi, A., Salama, A., Youssef, F., El-Ahmady, S., Ammar, N. (2022). 'Phytochemical Profile of Phoenix rupicola T. Anderson Seeds and In Vitro Evaluation of their Estrogenic Activity using MCF-7 Cell Lines', EKB Journal Management System, 65(132), pp. 1457-1464. doi: 10.21608/ejchem.2022.129433.5731
AbouZeid, E., Hussein, R., Afifi, A., Salama, A., Youssef, F., El-Ahmady, S., Ammar, N. Phytochemical Profile of Phoenix rupicola T. Anderson Seeds and In Vitro Evaluation of their Estrogenic Activity using MCF-7 Cell Lines. EKB Journal Management System, 2022; 65(132): 1457-1464. doi: 10.21608/ejchem.2022.129433.5731

Phytochemical Profile of Phoenix rupicola T. Anderson Seeds and In Vitro Evaluation of their Estrogenic Activity using MCF-7 Cell Lines

Egyptian Journal of Chemistry
Volume 65, Issue 132, December 2022, Pages 1457-1464    PDF (1011.22 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.129433.5731
Authors
Enaam AbouZeid* 1; Rehab Hussein2; Ahmed Afifi3; Abeer Salama4; Fadia Youssef5; Sherweit El-Ahmady6; Nagwa Ammar7
1Department of Pharmacognosy, Pharmaceutical and Drug Indutsries Research Institute, National Research Centre, Cairo, Egypt
2Pharmacognosy Department, National Research Centre, Dokki, Cairo, 12622, Egypt
3bPharmacognosy Department, Pharmaceutical and Drug Industries Research Division, National Research Centre
4Department of Pharmacology, National Research Centre
5Pharmacognosy Department, Faculty of Pharmacy, Ain Shams University. Cario, Egypt
6Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams Univeristy
7Department of Pharmacognosy, Pharmaceutical and Drug Industries Research Institute, National Research Centre
Abstract
The current study aimed to investigate the phytochemical profile and the in vitro estrogenic activity of Phoenix rupicola T. Anderson seeds’ extracts. Gas chromatography/ mass spectrometry (GC/MS) of the petroleum ether (seed oil) and chloroform extracts revealed the characterization of 93 compounds. γ-Sitosterol (8.54%), n-dodecane (6.00%), n-undecane (5.87%) and (2E)-2-decenal (3.48%) represented the major compounds in the petroleum ether extract, whereas lauric acid (21.73%), γ-sitosterol (21.21%), palmitic acid (9.19%) and myristic acid (6.37%) were the main components in the chloroform extract. Moreover, investigation of the 70% aqueous methanol extract by high-performance liquid chromatography coupled with diode-array detector (HPLC-DAD) revealed the identification of 13 phenolic compounds, of which catechin (105.75 mg%), vanillic acid (58.26%), p-hydroxybenzoic acid (32.84%) and cinnamic acid (27.62%) dominated The estrogenic activity of the extracts was tested in vitro via sulforhodamine B (SRB) assay on MCF-7 cells, where petroleum ether extract at a dose of 10 ug/mL exhibited the highest activity (p < 0.05) indicated by 1.17 % increase in cells’ proliferation compared to 17β-estradiol. Consequently, P. rupicola seeds provide a promising source of phytoconstituents with estrogenic activity worthy for further detailed studies.
Keywords
Phoenix rupicola seeds; GC/MS; HPLC-DAD; in vitro estrogenic assay; MCF-7 cell lines
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