Immunohistochemical Expression of CD44 and DNA Analysis in Oral Premalignant and Malignant Lesions | ||
| Egyptian Dental Journal | ||
| Article 7, Volume 68, Issue 3, July 2022, Pages 2265-2274 PDF (2.17 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/edj.2022.128820.2034 | ||
| Authors | ||
| Ahmed Hussein* 1; Mohamed Badawy2; Hany Gobran3; Hesham Dameer3; Hazem L. Abdel-Aleem4; Hend Abdel Hamid5 | ||
| 1Lecturer of Oral and Maxillofacial Pathology Department, Faculty of Dentistry, Assiut University, Assiut, Egypt. | ||
| 2Lecturer of Oral Biology Department, Faculty of Dentistry, Assiut University, Assiut, Egypt. | ||
| 3Associated Professor of Oral Biology Department, Faculty of Dentistry, Al-Azhar University, Cairo Branch, Egypt. | ||
| 4Lecturer of Dermatology, Andrology and Venereology Department, Faculty of Medicine, Al-Azhar University, Assiut, Egypt. | ||
| 5Lecturer of Oral Pathology Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt. | ||
| Abstract | ||
| Objectives: The aim of the present study is to evaluate CD44 expression to detect cancer stem cell activity along with analyzing the DNA ploidy, to predict the activity of oral carcinogenesis. Materials and method: Forty formalin fixed paraffin embedded tissues of oral lesions were used and divided equally into 4 groups; different premalignant white lesions, well, moderate, and poorly differentiated squamous cell carcinomas. All specimens were immunostained using CD44 antibodies. Moreover, the same paraffin blocks for each case used to prove the differentiation in DNA ploidy between groups using flow cytometer. Results: The CD44 immunoexpression showed a marked statistical significant difference between premalignant white lesions and poorly differentiated squamous cell carcinomas. Flow cytometric analysis of DNA parameters between the tested groups revealed a powerful difference of DNA ploidy between the premalignant group and other malignant groups. The S-phase fraction showed significant difference between poorly differentiated OSCC and premalignant lesions with no effective differences between the malignant lesions. Conclusions: The findings of the present study suggest that the CD44 immunoexpression collaborates with the DNA content analysis presented a real indicator in prediction the behavior of oral premalignant and malignant lesions. | ||
| Keywords | ||
| Cancer stem cell; CD44; DNA Ploidy; Premalignant lesions; OSCC | ||
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