Detection of Enterococcal asa1 and vanA Genes in Clinical Samples from Adult Immunocompromised Patients
Kamel, N., Ahmed, M., Salem, M., Azmy, A., Fahmy, E. (2020). Detection of Enterococcal asa1 and vanA Genes in Clinical Samples from Adult Immunocompromised Patients. EKB Journal Management System, 29(1), 133-138. doi: 10.21608/ejmm.2020.249870
Nesreen M. Kamel; Mai E. Ahmed; Mohamed N. Salem; Ahmed F. Azmy; Ehab M. Fahmy. "Detection of Enterococcal asa1 and vanA Genes in Clinical Samples from Adult Immunocompromised Patients". EKB Journal Management System, 29, 1, 2020, 133-138. doi: 10.21608/ejmm.2020.249870
Kamel, N., Ahmed, M., Salem, M., Azmy, A., Fahmy, E. (2020). 'Detection of Enterococcal asa1 and vanA Genes in Clinical Samples from Adult Immunocompromised Patients', EKB Journal Management System, 29(1), pp. 133-138. doi: 10.21608/ejmm.2020.249870
Kamel, N., Ahmed, M., Salem, M., Azmy, A., Fahmy, E. Detection of Enterococcal asa1 and vanA Genes in Clinical Samples from Adult Immunocompromised Patients. EKB Journal Management System, 2020; 29(1): 133-138. doi: 10.21608/ejmm.2020.249870

Detection of Enterococcal asa1 and vanA Genes in Clinical Samples from Adult Immunocompromised Patients

Egyptian Journal of Medical Microbiology
Volume 29, Issue 1, January 2020, Page 133-138  XML PDF (310.11 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2020.249870
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Authors
Nesreen M. Kamel* 1; Mai E. Ahmed1; Mohamed N. Salem2; Ahmed F. Azmy3; Ehab M. Fahmy4
1Department of Clinical Pathology
2Department of Internal Medicine Faculty of Medicine – Beni-Suef University, Beni-suef, Egypt
3Department of Microbiology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt
4Department of Medical Microbiology and Immunology, Aswan Faculty of Medicine, Aswan, Egypt
Abstract
Background: Vancomycin resistant Enterococci (VRE) is important opportunistic nosocomial pathogens due to rapid spread, and limited treatment options. Objectives: The aims of this study were to assess incidence of enterococcal infections and VRE in adult immunocompromised patients and detection of asa1 and vanA genes among these isolates. Methodology: The study included 80 adult immunocompromised patients admitted to Beni-Suef University Hospital. Antimicrobial susceptibility was done by disc diffusion method then MIC was done for vancomycin resistant strains and Enterococcus was considered VRE if the MIC was ≥ 16μg/ml. PCR was done for detection of vanA and asa1 genes. Results: Enterococci were detected in 36 samples, 22.2% of isolates were vancomycin resistant. vanA and asa1 were detected in 33.3% and 63.8% of isolates respectively. VanA gene was detected in 4 (50%) of VRE whereas vanA gene was detected in 4 VSE isolates. Comclusion: Further studies are needed for detection of other virulence and resistance genes and their role in bacterial pathogenicity.
Keywords
Immunocompromised; Enterococci; VRE; vanA; asa1
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