Prevalence of Helicobacter pylori cagA and vacA Genes and Their Correlation with Gastrointestinal Diseases
El-Sabbagh, A., Yassen, A., Abdelsalam, M. (2020). Prevalence of Helicobacter pylori cagA and vacA Genes and Their Correlation with Gastrointestinal Diseases. EKB Journal Management System, 29(4), 151-156. doi: 10.51429/EJMM29419
Amr M. El-Sabbagh; Ahmed H. Yassen; Maha M. Abdelsalam. "Prevalence of Helicobacter pylori cagA and vacA Genes and Their Correlation with Gastrointestinal Diseases". EKB Journal Management System, 29, 4, 2020, 151-156. doi: 10.51429/EJMM29419
El-Sabbagh, A., Yassen, A., Abdelsalam, M. (2020). 'Prevalence of Helicobacter pylori cagA and vacA Genes and Their Correlation with Gastrointestinal Diseases', EKB Journal Management System, 29(4), pp. 151-156. doi: 10.51429/EJMM29419
El-Sabbagh, A., Yassen, A., Abdelsalam, M. Prevalence of Helicobacter pylori cagA and vacA Genes and Their Correlation with Gastrointestinal Diseases. EKB Journal Management System, 2020; 29(4): 151-156. doi: 10.51429/EJMM29419

Prevalence of Helicobacter pylori cagA and vacA Genes and Their Correlation with Gastrointestinal Diseases

Egyptian Journal of Medical Microbiology
Volume 29, Issue 4, October 2020, Page 151-156  XML PDF (346.99 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.51429/EJMM29419
View on SCiNiTO View on SCiNiTO
Authors
Amr M. El-Sabbagh email 1; Ahmed H. Yassen2; Maha M. Abdelsalam3
1Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Egypt
2Tropical Medicine Department, Faculty of Medicine, Mansoura University, Egypt
3Clinical Pathology Immunology Department, Faculty of Medicine, Mansoura University, Egypt
Abstract
Background: Helicobacter pylori (H. pylori) is a gram-negative bacterium, producing gastric ulcer, mild to severe gastritis, gastric carcinoma and lymphoma to the gastric mucosa-associated lymphoid tissue through many virulence influences. Among the virulence factors identified; vacuolating cytotoxin A (vacA) and cytotoxin-associated gene A (cagA) play an important role. Objective: In this study, we try to discover the relation between vacA variant and cagA genes with the clinical illness occurring in H. pylori patients Methodology: One hundred and forty patients were included in our study. Dual biopsy samples were taken from the stomach; one was examined by the urease test, and the other one was stored at −80°C for DNA extraction followed by PCR. The existence of H. pylori in the tissue was recognized by the existence of glmM gene and its detection by PCR. All the positive samples were additionally tested by PCR for the occurrence of cagA and vacA variant genes. Results: Our study demonstrated that cagA and vacA genes were found among 50% and 57% respectively of H. pylori patients complaining from gastrointestinal illnesses and that vacA s1/s2 was the main genotype found in H. pylori persons with gastroduodenal disease. Significant relation between vacA s1 gene and cagA gene was found. Conclusion: vacA s1 genotype has a vital role in upper gastrointestinal illnesses progress.
Keywords
H. pylori; Peptic ulcer; Gastrointestinal Diseases; vacA variant and cagA
Statistics
Article View: 290
PDF Download: 185