MOLECULAR ANALYSIS OF INTERACTION PARTNERS OF THE TRANSCRIPTIONAL REPRESSOR BLIMP-1 IN DROSOPHILA MELANOGASTER BY A PROTEOMICS APPROACH. | ||||
| Al-Azhar Bulletin of Science | ||||
| Article 4, Volume 2014, Issue 6, June 2014, Page 35-42 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/absb.2014.25193 | ||||
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| Authors | ||||
| Moustafa Sarhan; Moustafa M. Sarhan* | ||||
| Zoology Department, Faculty of Science, Al Azhar, University, Assiut | ||||
| Abstract | ||||
| B lymphocyte induced maturation protein (Blimp-1) is present in a variety of organisms from nematode to human as a factor carrying well conserved five DNA binding zinc fingers and relatively conserved PR/SET domain. Blimp-1 was found to play important roles in various aspects of development in many organisms andworks mainly as a repressor to suppress many different genes. Blimp-1 inDrosophila was identified as a transcriptional repressor binds to the promoter region of the ftz-f1 gene. To understand the mechanism of how Drosophila Blimp-1 controls expression of its target genes, Blimp-1 was purified from prepared nuclear extract with its interacting proteins. The purified proteins were identified using Liquid chromatography-mass spectrometry (LC-MS/MS) after trypsin digestion. Results of this analysis revealed presence of several peptides of Histone H4 as interacting protein. It could be suggested that Blimp-1 function through a histone-masking mechanism and has an important regulatory chromatin remodeling. | ||||
| Keywords | ||||
| Drosophila; Blimp-1; Histone H4; Transcription factor; Gene regulation | ||||
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