Effect of Bioactive Glass Nano Particulates and Nano Hydroxyapatite Coated by Chitosan on Odontogenic Differentiation and Proliferation of human Dental Pulp Stem Cells | ||
| Egyptian Dental Journal | ||
| Article 25, Volume 68, Issue 3 - Serial Number 5, July 2022, Pages 2985-2994 PDF (2.38 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/edj.2022.139190.2113 | ||
| Authors | ||
| Hanan Gamal Ali* 1; Abeer Mahran2; Tariq Yehia3 | ||
| 1Endodontic Departement,Faculty of Dentistry Ain Shams University,Cairo.Egypt | ||
| 2Endodontic department ,Faculty Of Dentistry ,Ain Shams University | ||
| 3Endodontic Department, Faculty of Dentistry, Ain Shams University, Cairo, Egypt | ||
| Abstract | ||
| This study aimed to evaluate effect of Nano hydroxyapatite coated by Chitosan (NHAP/CS) and bioactive glass nano particulates (nBG) on odontogenic differentiation and proliferation of human dental pulp stem cells (hDPSCs). Methods: hDPSCs were seeded in direct contact with the corresponding Nano-biomaterials in 5 groups: Group (Ι): NHAP (10µg/ml), Group (ΙΙ): NHAP/CS (10µg/ml), Group (ΙΙΙ): nBG (500µg/ml). Group (IV): hDPSCs were cultured with osteogenic medium as a positive control while Group (V): hDPSCs were cultured with Dulbecco's Modified Eagle Medium (DMEM) only as a negative control. Odontogenic differentiation was evaluated based on mineralization related genes .Gene expressions were verified using alkaline phosphatase (ALP) assay and immunofluorescence staining of Dentin matrix protein (DMP-1). Proliferation of cells was evaluated via Trypan Blue staining test and methylthiazol tetrazolium (MTT) assay. Results: All the tested Nano biomaterials exhibited favorable media for odontogenic differentiation and proliferation of hDPSCs. NHAP/CS showed the highest proliferation index via MTT, highest ALP concentration and highest DMP-1Immunofluorescence staining. Conclusions: Compared to pure NHAP and a major biomaterial as nBG, NHAP/CS can induce odontogenic differentiation and proliferation of hDPSCs and could be considered as a good candidate for dentin-pulp regenerative procedures. | ||
| Keywords | ||
| Alkaline phosphatase; Dentin matrix; Immunofluorescence; Pulp; Regeneration | ||
|
Statistics Article View: 408 PDF Download: 325 |
||
