Molecular phylogeny and histopathological studies of Gotocotyla secundus (Monogenea: Polyopisthocotylea) isolated from Narrow-barred Spanish mackerel, Scomberomorous commerson in Suez Governorate, Egypt | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 41, Volume 26, Issue 4, July and August 2022, Page 687-704 PDF (1.04 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejabf.2022.253695 | ||||
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| Author | ||||
| Maather M. El-lamie et al. | ||||
| Abstract | ||||
| This study investigated the prevalence, molecular typing, as well as histopathological changes associated with Gotocotyla secundus infection in Scomberomorous commerson collected from Suez Governorate, Egypt. Identification of G. secundus was performed microscopically and genetically using C-PCR assay. Moreover, the phylogenetic tree was performed with MEGA X software analysis based on sequence analyses of the 28S rRNA gene using both neighbor–joining (NJ) and maximum-likelihood (ML) methods. The prevalence, abundance, and intensities of the parasite were calculated based on their collintary relationship with fish length and weight. Herein, G. secundus was mainly isolated from the gills and pseudobranchs of infested fish. Mostly, naturally infected fish have no pathognomonic lesions, except for a few cases that showed marbling of the gills. Histopathological studies revealed necrosis and fibrosis of the gill filaments with severe inflammatory reactions represented by the presence of lymphocytes and numerous giant cells. | ||||
| Keywords | ||||
| Phylogenetic tree; 28S rRNA; Monogeneasis; Scomberomorous commerson; Gotocotyla secundus | ||||
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