Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.
Abd El fadeel, S., Yassa, H., Rashed, L., Abd El Fattah, G. (2022). Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.. EKB Journal Management System, 3(3), 87-104. doi: 10.21608/ejmr.2022.253797
Sara Abd Eltawab Abd El fadeel; Hanan Dawood Yassa; Laila Ahmed Rashed; Gaber Hassan Abd El Fattah. "Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.". EKB Journal Management System, 3, 3, 2022, 87-104. doi: 10.21608/ejmr.2022.253797
Abd El fadeel, S., Yassa, H., Rashed, L., Abd El Fattah, G. (2022). 'Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.', EKB Journal Management System, 3(3), pp. 87-104. doi: 10.21608/ejmr.2022.253797
Abd El fadeel, S., Yassa, H., Rashed, L., Abd El Fattah, G. Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.. EKB Journal Management System, 2022; 3(3): 87-104. doi: 10.21608/ejmr.2022.253797

Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.

Egyptian Journal of Medical Research
Volume 3, Issue 3, July 2022, Page 87-104  XML PDF (1.75 MB)
Document Type: Original Article
DOI: 10.21608/ejmr.2022.253797
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Authors
Sara Abd Eltawab Abd El fadeel1; Hanan Dawood Yassaorcid 1; Laila Ahmed Rashed2; Gaber Hassan Abd El Fattah1
1Anatomy and Embryology department,Faculty of Medicine, Beni-Suef University.
2Biochemistry department, Faculty of Medicine, Cairo University.
Abstract
Thirty five adult male albino rats were used in this study. They were divided into 7 groups: Group I (Control), Group II (low dose Zno NPs group) , Group III (medium dose Zno NPs group) , Group IV (high dose Zno NPs group), Group V (low dose Zno NPs-selenite group), Group VI (medium dose Zno NPs-selenite group), Group VII (high dose Zno NPs-selenite group): tissue of the spleen was examined histopathologically and immunohistochemical , Determination of blood cells count (number of white blood cells (WBCs), red blood corpuscles (RBCs) and platelets. Also tissue level of Hydrogen peroxide (H2O2) and paraoxonase-1(pon-1): for detection of oxidative stress estimated for all groups, PCR detection of apoptosis inducing factor (AIF), apoptotic protease activating factor-1 and caspases 3 and Zinc level in the spleen.
Results of the present study showed that, compared to controls: orally intake of Zinc oxide nanoparticles (Zno NPs) cause marked changes in spleen tissues as thickening of capsule, thickening of trabeculae , destruction of white pulp and red pulp expansion especially in groups receiving zinc oxide nanoparticles only and these changes markedly decreased on groups receiving selenium as protection
Keywords
Nanoparticles; ZnO NPs; selenium
References
  1. Boverhof, D. R., Bramante, C. M., Butala, J. H., Clancy, S. F , Lafranconi, M. et al. (2015): Comparative assessment of nanomaterial definitions and safety evaluation considerations. Regul. Toxicol. Pharmacol. 73, 137–150
  2. Eastlake, A., Zumwalde, R., and and Geraci, C. (2016): Can control banding be use-ful for the safe handling of nanomaterials? A systematic review. J. Nanopar-ticles Res. 18, 169. Pii
  3. Sapsford, K. E., Algar, W. R., Berti, L., Gemmill, K. B., Casey, B. J., et al(2013): Functionalizing nanoparticles with biological molecules: developing chemistries that facilitate nanotechnology. Chem. Rev. 113, 1904–2074.
  4. Yokel, R. A. and MacPhail, R. C. (2011): Engineered nanomaterials: expo-sures, hazards, and risk prevention. J. Occupat. Med. Toxicol.
  5. Cho, W. S., Duffin, R., and Thielbeer, F. (2012): Zeta potential and solubility to toxic ions as mechanisms of lung inflammation caused by metal/metal oxide nanoparticles. Toxicol. Sci. 126, 469–477 .
  6. Mansouri, E., Khorsandi, L., Orazizadeh, M., and Jozi, Z. (2015): Dose-dependent hepatotoxicity effects of Zinc oxide nanoparticles. Nanomed. J. 2, 273-282
  7. Gojova, A., Guo, B., Kota, R. S., Rutledge, J. C., Kennedy, I. M., et al. (2007): Induction of inflammation in vascular endothelial cells by metal oxide nano-particles: effect of particle composition. Environ. Health 115, 403,409
  8. Tapiero, H, Townsend, D.M and Tew, K.D(2003): The antioxidant role of selenium and seleno-compounds. Biomed Pharm.;57:134–44
  9. Pandurangan, M., and Kim, D. H. (2015): In vitro toxicity of zinc oxide nano-particles: a review. J. Nanoparticle Res. 17, 1–8
  10. Sharma, V., Singh, P., Pandey, A. K. and Dhawan, A. (2012): Induction of oxidative stress, DNA damage and apoptosis in mouse liver after sub-acute oral exposure to zinc oxide nanoparticles. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 745(1), 84-91.
  11. Viezeliene, D., Jansen, E., Rodovicius, H., Kasauskas, A. and Ivanov, L. (2011): Protective effect of selenium on aluminium-induced oxidative stress in mouse liver in vivo. environmental toxicology and pharmacology, 31(2), 302-306.
  12. Bancroft, J. D., & Gamble, M. (Eds.). (2018): Theory and practice of histological techniques. Elsevier health sciences.
  13. Winiarska-Mieczan, A. (2018): Protective effect of tea against lead and cadmium-induced oxidative stress—a review. Biometals, 31(6), 909-926.
  14. Smijs, T. G., & Pavel, S. (2011): Titanium dioxide and zinc oxide nanoparticles in sunscreens: focus on their safety and effectiveness. Nanotechnology, science and applications, 4, 95.
  15. Ruszkiewicz, J. A., Pinkas, A., Ferrer, B., Peres, T. V., Tsatsakis, A., & Aschner, M. (2017): Neurotoxic effect of active ingredients in sunscreen products, a contemporary review. Toxicology Reports, 4, 245-259.
  16. M Herranz, L., Teba, F., Martin, R., Ingelmo, I., Gomez, V., Codesal, J., & Santamaría, L. (2010): Quantitative changes in rat seminiferous epithelium after chronic administration of low doses of cadmium and zinc: A stereological study. The Open Andrology Journal, 2(1).
  17. Wluka, A., & Olszewski, W. L. (2006): Innate and adaptive processes in the spleen. Annals of transplantation, 11(4), 22-29.
  18. Mohamed, H. Z., Ragab, I. K., & Ghafeer, H. H. (2016): A histological study on the possible protective effect of selenium against chromium-induced thyrotoxicity in adult male albino rats. The Egyptian Journal of Histology, 39(1), 1-11.
  19. Wang, B., Feng, W., Wang, M., Wang, T., Gu, Y., Zhu, M., & Chai, Z. (2008): Acute toxicological impact of nano-and submicro-scaled zinc oxide powder on healthy adult mice. Journal of Nanoparticle Research, 10(2), 263-276.
  20. Mansouri, E., Khorsandi, L., Orazizadeh, M., & Jozi, Z. (2015): Dose-dependent hepatotoxicity effects of zinc oxide nanoparticles.
  21. Ebaid, H. M., & Tag, H. M. (2012): Monosodium glutamate toxic effect on spleen structure and potentiality of recovery in adult Albino rats. Egyptian Academic Journal of Biological Sciences, B. Zoology, 4(1), 1-8.
  22. Al-Rasheed, N. M., Baky, N. A., Faddah, L. M., Fatani, A. J., Hasan, I. H., & Mohamad, R. A. (2014): Prophylactic role of a-lipoic acid and vitamin E against zinc oxide nanoparticles induced metabolic and immune disorders in rat’s liver. Eur Rev Med Pharmacol Sci, 18(12), 1813-1828.
  23. Wang, B., Feng, W. Y., Wang, M., Shi, J. W., Zhang, F., Ouyang, H., & Wang, H. F. (2007): Transport of intranasally instilled fine Fe 2 O 3 particles into the brain: micro-distribution, chemical states, and histopathological observation. Biological trace element research, 118(3), 233-243.
  24. Zerjatke, T., Gak, I. A., Kirova, D., Fuhrmann, M., Daniel, K., Gonciarz, M & Mansfeld, J. (2017): Quantitative cell cycle analysis based on an endogenous all-in-one reporter for cell tracking and classification. Cell reports, 19(9), 1953-1966.
  25. Kanter, M., Aktas, C., & Erboga, M. (2013): Curcumin attenuates testicular damage, apoptotic germ cell death, and oxidative stress in streptozotocin‐induced diabetic rats. Molecular nutrition & food research, 57(9), 1578-1585.
  26. Lane, D. P. (1992): Cancer. p53, guardian of the genome. Nature, 358, 15-16.
  27. Ng, K. W., Khoo, S. P., Heng, B. C., Setyawati, M. I., Tan, E. C., Zhao, X., ... & Loo, J. S. (2011): The role of the tumor suppressor p53 pathway in the cellular DNA damage response to zinc oxide nanoparticles. Biomaterials, 32(32), 8218-8225.
  28. Meyer, K., Rajanahalli, P., Ahamed, M., Rowe, J. J., & Hong, Y. (2011): ZnO nanoparticles induce apoptosis in human dermal fibroblasts via p53 and p38 pathways. Toxicology in vitro, 25(8), 1721-1726.
  29. Chaudhary, S., Umar, A., & Mehta, S. K. (2016): Selenium nanomaterials: an overview of recent developments in synthesis, properties and potential applications. Progress in Materials Science, 83, 270-329.
  30. Burk, R. F., & Hill, K. E. (1993): Regulation of selenoproteins. Annual review of nutrition, 13(1), 65-81
  31. Yu, K. N., Yoon, T. J., Minai-Tehrani, A., Kim, J. E., Park, S. J., Jeong, M. S., & Cho, M. H. (2013): Zinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generation. Toxicology in Vitro, 27(4), 1187-1195.
  32. Ng, A. M. C., Chan, C. M. N., Guo, M. Y., Leung, Y. H., Djurišić, A. B., Hu, X., ... & Tong, S. Y. (2013): Antibacterial and photocatalytic activity of TiO 2 and ZnO nanomaterials in phosphate buffer and saline solution. Applied microbiology and biotechnology, 97(12), 5565-5573.
  33. Zhang, X. D., Di Wu, X. S., Liu, P. X., Yang, N., Zhao, B., Zhang, H., ... & Fan, F. Y. (2011): Size-dependent in vivo toxicity of PEG-coated gold nanoparticles. International journal of nanomedicine, 6, 2071.
  34. Chen, L. Q., Fang, L., Ling, J., Ding, C. Z., Kang, B., & Huang, C. Z. (2015): Nanotoxicity of silver nanoparticles to red blood cells: size dependent adsorption, uptake, and hemolytic activity. Chemical research in toxicology, 28(3), 501-509.
  35. Liu, Z. H., Wang, X., Wang, H. F., Wang, T. C., Gu, Y. Q., Yan, L., & Zang, J. J. (2008): Actute toxicity of nano-sized Zn oxide in ICR mice via intratracheal instillation. J Environ Occup Med, 25(4), 360-364.
  36. Li, C. H., Liao, P. L., Shyu, M. K., Liu, C. W., Kao, C. C., Huang, S. H., ... & Kang, J. J. (2012): Zinc oxide nanoparticles–induced intercellular adhesion molecule 1 expression requires Rac1/Cdc42, Mixed Lineage Kinase 3, and c-Jun N-Terminal kinase activation in endothelial cells. Toxicological Sciences, 126(1), 162-172.
  37. Park, H. S., Kim, S. J., Lee, T. J., Kim, G. Y., Meang, E., Hong, J. S., & Kang, J. S. (2014): A 90-day study of sub-chronic oral toxicity of 20 nm positively charged zinc oxide nanoparticles in Sprague Dawley rats. International journal of nanomedicine, 9(Suppl 2), 93.
  38. Molnár, T., Biró, J., Balogh, K., Mézes, M., & Hancz, C. (2012): Improving the nutritional value of Nile tilapia fillet by dietary selenium supplementation. Israeli Journal of Aquaculture – Bamidgeh, IJA_64.2012.744, 744– 750.
  39. El-Hammady, A., El-Kasheif, M., & Ibrahim, S. (2007): SYNERGISTIC REACTIONS BETWEEN VITAMIN EAND SELENIUM IN DIETS OF HYBRID TILAPIA (OREOCHROMIS NILOT1CUS X OREOCHROMIS AUREUS) AND THEIR EFFECT ON THE GROWTH AND LIVER HISTOLOGICAL STRUCTURE. Egyptian Journal of Aquatic Biology and Fisheries, 11(1), 53-81.
  40. Khan, K. U., Zuberi, A., Nazir, S., Fernandes, J. B. K., Jamil, Z., & Sarwar, H. (2016): Effects of dietary selenium nanoparticles on physiological and biochemical aspects of juvenile Tor putitora. Turkish Journal of Zoology, 40(5), 704-712.
  41. Kao, Y. Y., Chen, Y. C., Cheng, T. J., Chiung, Y. M., & Liu, P. S. (2012): Zinc oxide nanoparticles interfere with zinc ion homeostasis to cause cytotoxicity. Toxicological Sciences, 125(2), 462-472.
  42. Rahman, H. S., Rasedee, A., Abdul, A. B., Zeenathul, N. A., Othman, H. H., Yeap, S. K., ... & Hafiza, W. A. G. W. N. (2014): Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line. International journal of nanomedicine, 9, 527.
  43. Curtin, J. F., Donovan, M., & Cotter, T. G. (2002): Regulation and measurement of oxidative stress in apoptosis. Journal of immunological methods, 265(1-2), 49-72.
  44. Nazarizadeh, A., & Asri-Rezaie, S. (2016): Comparative study of antidiabetic activity and oxidative stress induced by zinc oxide nanoparticles and zinc sulfate in diabetic rats. AAPS PharmSciTech, 17(4), 834-843.
  45. Yamamoto, O. (2001): Influence of particle size on the antibacterial activity of zinc oxide. International Journal of Inorganic Materials, 3(7), 643-646.
  46. Song, W., Zhang, J., Guo, J., Zhang, J., Ding, F., Li, L., & Sun, Z. (2010): Role of the dissolved zinc ion and reactive oxygen species in cytotoxicity of ZnO nanoparticles. Toxicology letters, 199(3), 389-397.
  47. Hussein, J., El-Banna, M., Razik, T. A., & El-Naggar, M. E. (2018): Biocompatible zinc oxide nanocrystals stabilized via hydroxyethyl cellulose for mitigation of diabetic complications. International journal of biological macromolecules, 107, 748-754.
  48. Zhao, J., Bowman, L., Zhang, X., Shi, X., Jiang, B., Castranova, V., & Ding, M. (2009): Metallic nickel nano-and fine particles induce JB6 cell apoptosis through a caspase-8/AIF mediated cytochrome c-independent pathway. Journal of nanobiotechnology, 7(1), 2.
  49. Jing, Y., Dai, J., Chalmers-Redman, R. M., Tatton, W. G., & Waxman, S. (1999): Arsenic trioxide selectively induces acute promyelocytic leukemia cell apoptosis via a hydrogen peroxide-dependent pathway. Blood, the Journal of the American Society of Hematology, 94(6), 2102-2111.
  50. Sabour‐Pickett, S., Nolan, J. M., Loughman, J., & Beatty, S. (2012): A review of the evidence germane to the putative protective role of the macular carotenoids for age‐related macular degeneration. Molecular nutrition & food research, 56(2), 270-286.
  51. Faddah, L. M; Adbel Baky, N. A; Mohamed, A. M; Al-Rasheed, N. M; Al-Rasheed, N. M. J. (2013): Nanopart. Res. 15:1520.
 

 

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