Detection of aflatoxins in meat by modified HPLC method | ||||
| Egyptian Journal of Chemistry and Environmental Health | ||||
| Volume 1, Issue 1, November 2015, Page 945-954 PDF (235.13 K) | ||||
| Document Type: Scientific and Research | ||||
| DOI: 10.21608/ejceh.2015.253978 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mai Abdel Monem* ; Marwa Ragab; M. Ahmed Maher; Shaimaa H. Ali; Nahla Salah; Heba M. Hassan; Fatma H. Amro | ||||
| Reference laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institutes, Dokki | ||||
| Abstract | ||||
| An analytical method for the determination of aflatoxins in meat was developed. Samples prepared by an extraction with dichloromethane followed by a solid phase cleanup and then fluorescence detection. Separation of the four aflatoxins(B1,G1,B2 and G2) with higher selectivity and sensitivity, and within reasonable limits of retention time, was performed. The isocratic mobile phase of water/methanol/acetonitrile (60/20/20) was used at a column temperature of 30C,with flow rate 1ml/minute and injection volume 20µl. Optimization of samples preparation and analytical conditions gave recoveries in the range of 80–90 % at spike levels of 5-40 ug/kg, and relative standard deviations (RSDs) lower than 1% in all the cases. Additionally, the limit of detection (LOD) (5ug/kg) and the limit of quantification (LOQ) (10 ug/kg) of these aflatoxins are within the legal limits set by the European Union (EU,2006). | ||||
| Keywords | ||||
| Aflatoxins; validation; HPLC | ||||
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