Isolation and identification of Sphingomonas paucimobilis 503: a case report | ||||
Damanhour Journal of Veterinary Sciences | ||||
Volume 9, Issue 2, February 2023, Page 17-20 PDF (297.91 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/djvs.2022.142767.1078 | ||||
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Authors | ||||
Noha Awwad1; Dalia Talat1; Hany Ellakany2; Madiha Salah Ibrahim ![]() ![]() | ||||
1Department of Microbiology, Faculty of Veterinary Medicine, Damanhour University, Egypt | ||||
2Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Egypt | ||||
Abstract | ||||
In this study, the bacterium Sphingomonas sphingomonas 503 was isolated from water samples. One ml of water sample was cultured on MH broth for enriching bacterial growth then subcultured on MH agar plates till obtaining pure separate deep yellow-pigmented colonies (after four subcultures). The bacteria were cultured on blood agar and chromogenic agar, giving white hemolytic and green colonies, respectively. The bacterial isolates showed Gram-negative, polymorphic rods without special arrangement. The bacteria was identified by the VITEK Compaq® 2 system as Sphingomonas paucimobilis 503 and assayed for antibiotic susceptibility using Ampicillin, Ampicillin/sulbactam, Piperacillin/tazobactam, Cefazolin, Ceftazidime, Ceftriaxone, Cefepime, Meropenem, Amikacin, Gentamycin, Tobramycin, Ciprofloxacin, Levofloxacin, Nitrofurantoin, Trimethoprim/Sulfamethazole. This bacterium was sensitive to all tested antibiotics except Ceftazidime, which showed the highest MIC ≥ 64, while the lowest MIC was that of Levofloxacin ≤0.12and Ciprofloxacin ≤ 0.25 | ||||
Keywords | ||||
S. paucimobilis 503; Biochemical identification; Antimicrobial susceptibility | ||||
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