Characterization of amylase-producing Streptomyces sp. NAA-28 isolated from mangrove sediment, Red Sea, Egypt | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Article 62, Volume 26, Issue 4, July and August 2022, Pages 1053-1065 PDF (940.17 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2022.256201 | ||
| Author | ||
| Nayer M. Fahmy | ||
| Abstract | ||
| Hydrolytic enzymes produced by actinomycetes are of outstanding quality and have important industrial uses. Streptomyces sp. NAA-28, an amylase-producing bacterium, was isolated from mangrove sediments and was chosen for comprehensive characterization and optimization of amylase production, based on its higher amylolytic activity as determined by the formation of a clear zone using starch agar medium. The strain was identified based on the biochemical and morphological characteristics and the sequence of the 16s rDNA gene. The sequence was deposited in GenBank under the accession number MZ496703. The optimum NaCl concentration for amylase production was 6%. The strain produced the highest amount of amylase after 5 days of incubation at 35°C in the culture medium adjusted to pH 8 and supplemented with peptone and starch as carbon and nitrogen sources, respectively. Further studies are required to purify and characterize the secreted enzyme to determine its potential for industrial applications. | ||
| Keywords | ||
| Streptomyces; mangrove; Red Sea; amylase; optimization | ||
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