ETHANOL PRODUCTION FROM CELLULOSE VIA INTERGENERIC PROTOPLAST FUSION BETWEEN Trichoderma reese; AND Saccharomyces cerevisiae FUSANTS | ||
| Journal of Plant Production | ||
| Article 9, Volume 27, Issue 11, November 2002, Pages 7335-7345 PDF (11.96 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2002.257032 | ||
| Authors | ||
| Fatma N. Talkhan,; A. M. EI-Bondkly; A. A. Ismail | ||
| Genetics and Cytology Department, National Research Center, Dokki, Cairo, Egypt. | ||
| Abstract | ||
| The present study aimed to investigate the possibility of inducing new genetic recombinants having the ability to ferment cellulose into ethanol upon fusion of T. reese; and S. cerevisiae protoplasts. Twelve stable fusants were induced and lested for ethanol as well as cellulase enzymes productivity. Out of these fusants 8 isolates produced ethanol with amounts ranged from 45% (F/4 and F/5) to 90% (Ff7) of the parent S. cerevisiee, whereas the remaing 4 did not produce ethanol. Meantime Ihese none ethanol producing fusants showed cellulase activities of the three enzymes (FPase, CMCase and ~-9Iucosidase). On th other hand, 3 fusants (F/1, F/6 and Fn) out of the eight ethanol producer showed cellulase activities of these three enzymes, whereas the other five fusants (F/2, F/4, F/5, F/9 and F/10) showed cellulase activities of the two enzymes FPar.e and CMCase only. None of these 12 fusants overyieled the parental strain T. reese; (3126) in cellulases production. | ||
| Keywords | ||
| Trichoderma reesei; Saccharomyces cerevisiae Cellulase enzymes; Ethanol; Intergeneric cross; Protoplast fusion | ||
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