Production and Optimization of Bioflocculant isolated from bacteria | ||
| Benha Journal of Applied Sciences | ||
| Article 28, Volume 7, Issue 4, April 2022, Pages 223-229 PDF (951.37 K) | ||
| Document Type: Original Research Papers | ||
| DOI: 10.21608/bjas.2022.257798 | ||
| Authors | ||
| Mervat.G. Hassan1; M.E. El Awady2; Sahar.S. Mohamed2; H.W. Elbanna1; M.O. Abdel-Monem1 | ||
| 1Botany and Microbiology Department, Faculty of science, Benha University, 13518 Benha,Egypt | ||
| 2Microbial Biotechnology Department, National Research Centre, El-Buhouth St. 33, Dokki-Cairo 12622, Egypt | ||
| Abstract | ||
| Bacterial extracellular polymeric substances (EPS) contribute to the structure, surface charge, and settling properties of activated sludge by assisting in the development of bioflocs. From various soil sources, a total of twenty bacterial isolates were collected. The maximum bacterial isolate was (HS9), which produced (5.8 g/l) after being examined for exopolysaccharide (EPS) synthesis. As a result, EPSs were selected based on Bio-flocculant activity, while HS9 was the most active (86.95 %). The lowest was HS1, while the highest was HS2 (14.55 %). The synthesis and production of EPS are influenced by a variety of variables. As a result, by examining the effects of various factors on the production of EPSs by isolate (HS9), it was discovered that the maximum yield of EPS was (7.9 g/l) and cell dry was (4.1 g/l) when incubation temperature was 40 °C after three days, RPM 120, pH 7, peptone as a nitrogen source, and sucrose (20 g/l) as a carbon source. | ||
| Keywords | ||
| Exopolysaccharides; Optimization; Production; Bacteria; Bioflocculant | ||
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