Enhancement of Growth, Lipid, and Carbohydrate Production of the Egyptian isolate Dunaliella salina SA20 Using Mozzarella Cheese Whey as a Growth Supplement | ||
Egyptian Journal of Botany | ||
Article 7, Volume 63, Issue 1, January 2023, Pages 101-111 PDF (1.03 M) | ||
Document Type: Regular issue (Original Article) | ||
DOI: 10.21608/ejbo.2022.145428.2020 | ||
Authors | ||
Hend Y. Mohammad1; Fatma El-Zahraa Tawfeek1; Eladl Eltanahy2; Tamer A. Mansour3; Zeinab Khalil* 1 | ||
1Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, Egypt | ||
2Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt | ||
3Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt | ||
Abstract | ||
THE growth of Dunaliella salina SA20 (Dunal) Teodoresco Egyptian isolate (from El-Bardawil Lake, Sinai, Egypt) in wastewater is a more cost-effective cultivation method. Wastewater, such as mozzarella cheese whey (MCW), is produced in massive quantities as a dairy industry byproduct, and growing D. salina in such waste could be used safely in biotechnological applications. Applying different organic MCW ratios to inorganic Loeblich nutrient medium (20, 50, 75% v/v MCW/Loeblich) as growth supplement allowed mixotrophic nutrition, while growth in completely inorganic medium (100% Loeblich medium) allowed for autotrophic nutrition. The optimum growth performance of D. salina cells was observed in 20% MCW and was indicated by measured values of chlorophyll a (10.51mg/L), dry weight (405mg/L), cell count (6.92 x 106cell/ml), and optical density (1.12). Using 20% MCW, different pH values were applied to explore the pH effect on nutrient availability, and pH 7.5 was found to be optimal for growth. Additionally, a growth curve experiment comparing the mix- and autotrophic conditions presented higher values of the relative mean and maximum growth rates in mixotrophic conditions (0.19, 0.21 day−1, respectively) than in the autotrophic conditions (0.16, 0.18 day−1, respectively). | ||
Keywords | ||
Dunaliella salina; Growth supplements; Mixotrophy; Whey | ||
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