Effect of titanium dioxide nanoparticles on the spleen of adult male albino rats: Histological and immunohistochemical study | ||||
| Egyptian Journal of Histology | ||||
| Article 7, Volume 41, Issue 3, September 2018, Page 311-328 PDF (14.28 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/EJH.2019.25408 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Rania Ibrahim; Mohamed Y. Salem; Omayma K. Helal; Sahar N. Abd El-Monem | ||||
| Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha, Egypt. | ||||
| Abstract | ||||
| ABSTRACT Introduction: Titanium dioxide nanoparticles (TiO2 NPs) are commonly used in industry (e.g. cosmetics, sunscreens, food products, paints and drugs). But, they have different adverse cellular effects including oxidative stress and cellular damage. Aim of the work: To evaluate the possible toxic effect of titanium dioxide nanoparticles on the structure of the spleen of rats. Materials and Methods: Forty-five adult male rats were divided into three groups. Group I served as the control group. Group II (low-dose group) rats were given oral dose of TiO2 (600 mg/kg/day) daily for 8 weeks. Group III (high-dose group) rats were given a daily oral dose of TiO2 (1200 mg/kg/day) for 8 weeks. Spleen specimens were taken after 4 weeks, 8 weeks and 12 weeks from the start of the experiment. Thereafter, spleen specimens were processed for histological and immunohistochemical examinations. Results: The low-dose group showed slight disturbance in architecture of white pulp, slight congestion in red pulp and significant increase (P< 0.01) in CD4 and CD68 immuno-expression only after 8 week specimens compared with control group. The high-dose group showed marked disturbance in architecture of white pulp and significant increase (P< 0.01) in CD4 and CD68 immuno-expression after 4 week specimens, CD4 and CD68 immuno-expression significantly decreased (P< 0.01) in the 8 and in the 12 week specimens. No obvious improvement occurred after 12 weeks (withdrawal specimens). Conclusion: Prolonged exposure to daily high oral dose of TiO2 NPs has deleterious and toxic effects on the spleen of rat, besides disruption of its structure. | ||||
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