SURGICAL CREATION OF CLEFT-LIKE PALATAL DEFECTS IN NEW ZEALAND RABBITS (A PILOT STUDY)
Hamdy, A., Saad, M., Nouh, S., ElAshwah, A., El Mahallawy, A., Zaky, S., ElBackly, R. (2022). SURGICAL CREATION OF CLEFT-LIKE PALATAL DEFECTS IN NEW ZEALAND RABBITS (A PILOT STUDY). EKB Journal Management System, 47(3), 8-13. doi: 10.21608/adjalexu.2021.69140.1174
Ayat Hamdy; Manal Saad; Samir Rashed Nouh; Adham ahmed ElAshwah; Ahmed Salah El Mahallawy; Samer Zaky; Rania Mahmoud ElBackly. "SURGICAL CREATION OF CLEFT-LIKE PALATAL DEFECTS IN NEW ZEALAND RABBITS (A PILOT STUDY)". EKB Journal Management System, 47, 3, 2022, 8-13. doi: 10.21608/adjalexu.2021.69140.1174
Hamdy, A., Saad, M., Nouh, S., ElAshwah, A., El Mahallawy, A., Zaky, S., ElBackly, R. (2022). 'SURGICAL CREATION OF CLEFT-LIKE PALATAL DEFECTS IN NEW ZEALAND RABBITS (A PILOT STUDY)', EKB Journal Management System, 47(3), pp. 8-13. doi: 10.21608/adjalexu.2021.69140.1174
Hamdy, A., Saad, M., Nouh, S., ElAshwah, A., El Mahallawy, A., Zaky, S., ElBackly, R. SURGICAL CREATION OF CLEFT-LIKE PALATAL DEFECTS IN NEW ZEALAND RABBITS (A PILOT STUDY). EKB Journal Management System, 2022; 47(3): 8-13. doi: 10.21608/adjalexu.2021.69140.1174

SURGICAL CREATION OF CLEFT-LIKE PALATAL DEFECTS IN NEW ZEALAND RABBITS (A PILOT STUDY)

Alexandria Dental Journal
Article 2, Volume 47, Issue 3, December 2022, Page 8-13  XML PDF (685.92 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2021.69140.1174
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Authors
Ayat Hamdy email orcid 1; Manal Saadorcid 2; Samir Rashed Nouhorcid 3; Adham ahmed ElAshwahorcid 4; Ahmed Salah El Mahallawyorcid 5; Samer Zakyorcid 6; Rania Mahmoud ElBacklyorcid 7
1Dentist at Ministry of Health, Alexandria, Egypt and Researcher at Tissue Engineering Labs,Alexandria,Egypt.
2Professor of Oral Biology,Faculty of Oral and Dental Medicine, Ahram Canadian University, Giza, Egypt and Researcher Tissue Engineering laboratories, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
3Veterinary Surgery, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt and Researcher at Tissue Engineering laboratories, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
4Oral and maxillofacial surgery department, faculty of dentistry , Alexandria university , Alexandria ,Egypt
5Oral and Maxillofacial Surgery, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
6Center for Craniofacial Regeneration (CCR), University of Pittsburgh, Pennsylvania, USA
7Endodontics, Conservative Dentistry, Faculty of Dentistry, Alexandria University, Alexandria, Egypt and Researcher at Tissue Engineering laboratories, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
Abstract
Introduction: Surgically Created cleft palate models in animals have solved most of the problems associated with congenital models. Recent studies have preferred the use of rats and rabbits as they are easy to handle and observe.
Objective:. The aim of this study was to surgically create a palatal defect in white New Zealand in rabbits. These defects were assessed radiographically and histologically to select the most reliable, reproducible and least invasive dimensions for creating a cleft-like palatal defect suitable to be used in future research to assess the regenerative capacity of new materials for cleft palate management.
Materials and Methods: A total of 7 rabbits were used in this pilot study; for defect optimization. Three different defect dimensions 7×2.5×1 mm³, 7×2.5×5 mm³ and 5×2.5×4 mm³ were created in the mid-palatal region. Maxillae were taken immediately after surgery and at 2 weeks interval for radiographic and histological analyses.
Results: 7×2.5×1 mm³ defects were shallow such that spontaneous healing of defects occurred. 7×2.5×5 mm³ defects with nasal mucosa removal showed death of all animals with severe injuries in vital structures. On the other hand, 5×2.5×4 mm³ defects with intact nasal mucosa showed preservation of vital structures without closure of the defect at 2 weeks.
Conclusions:. 5×2.5×4 mm³ defects have been found to be the largest yet most preservative dimensions for creating palatal defects in that region in rabbits without allowing spontaneous healing till 2 weeks.
Keywords
Palatal defects; Cleft palate; Bone engineering; Rabbit model; Cleft animal model
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