11β Hydroxysteroid dehydrogenase type 1 Gene Expression in Obesity and its Complications | ||
| Egyptian Journal of Chemistry | ||
| Volume 66, Issue 7, July 2023, Pages 165-174 PDF (412.31 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2022.157145.6819 | ||
| Authors | ||
| Hend Aziz1; Mai Raslan2; Dina El Dahshan3; Mie Afify4; Waleed I. Hamimy5; Weaam Gouda* 6 | ||
| 1Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt. | ||
| 2Biotechnology and Life Sciences Department, Faculty of Postgraduate studies for Advanced Sciences, Beni-Suef University, Egypt. | ||
| 3Clinical Pathology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt. | ||
| 4Biochemistry Department, Biotechnology Research Institute, National Research Centre, Egypt | ||
| 5Department of Anesthesia, Surgical ICU and Pain Management, Faculty of Medicine, Cairo University, Cairo, Egypt. | ||
| 6Biochemistry department, Biotechnology Research Institute, National Research Centre, Egypt. | ||
| Abstract | ||
| ABSTRACT Background: 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) is an enzyme greatly expressed in the adipose tissue and acts as a reductase (convert inactive cortisone into active cortisol). Previous study assumed that the pathophysiology of obesity might be elaborated by tissue-specific dysregulation of cortisol metabolism. Numerous studies have assessed 11β-HSD1 gene polymorphisms and obesity risk. However, there is still a lack in the published literature evaluating the association between 11β-HSD1 gene expression and the risk of obesity and its related diseases. Objective: This study was carried out to investigate the relationship of 11β-HSD1 gene expression with obesity, diabetes, and hypertension in subcutaneous adipose tissue. Moreover, this study correlates 11β-HSD1 gene expression with various parameters in obese patients. Patients and methods: Our study was conducted on 74 subjects; they were selected from kasr Al Aini Hospital, Surgical Department. Obese patients (n=56) with BMI ≥30 kg/m2 were divided according to obesity complications into obese (n=26), obese hypertensive (n=14) and obese diabetic (n=16) groups beside 18 age- and sex-matched normal weight healthy subjects as control group. The expression of 11β-HSD1 was evaluated with real-time PCR. Results: There was a significant difference between obese and controls in HSD11β1 gene expression level and we found a significant difference in obese patients with diabetes and hypertension compared to normal weight healthy controls. Furthermore, the correlation and regression analysis showed that the 11β-HSD1 gene expression was correlated with fasting blood glucose and triglycerides levels in obese patients. Conclusion: The study shows a significant association between subcutaneous adipose tissue 11β-HSD1 gene expression and obesity. Moreover, the subcutaneous adipose tissue expression level of 11β-HSD1 gene was correlated to fasting plasma glucose level and also correlated to triglycerides. So the 11β-HSD1 gene expression is significantly increased the risk of diabetes mellitus and hypertension in obese individuals. | ||
| Keywords | ||
| Keywords: 11β -HSD1; gene expression; obese; diabetes mellitus; hypertension | ||
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