Antimicrobial and Anti-Biofilm Activities of Bee Venom against some Dental pathogens
Mostafa, H., Masry, S., Abou-Zeid, M., Abd El Wahab, A., El Demellawy, M., Abdou, D. (2022). Antimicrobial and Anti-Biofilm Activities of Bee Venom against some Dental pathogens. EKB Journal Management System, 60(3), 1-16. doi: 10.21608/ejaps.2022.142059.1035
Haitham Mostafa; Saad Masry; Mohamed Abou-Zeid; Abeer Abd El Wahab; Maha El Demellawy; Dalia Abdou. "Antimicrobial and Anti-Biofilm Activities of Bee Venom against some Dental pathogens". EKB Journal Management System, 60, 3, 2022, 1-16. doi: 10.21608/ejaps.2022.142059.1035
Mostafa, H., Masry, S., Abou-Zeid, M., Abd El Wahab, A., El Demellawy, M., Abdou, D. (2022). 'Antimicrobial and Anti-Biofilm Activities of Bee Venom against some Dental pathogens', EKB Journal Management System, 60(3), pp. 1-16. doi: 10.21608/ejaps.2022.142059.1035
Mostafa, H., Masry, S., Abou-Zeid, M., Abd El Wahab, A., El Demellawy, M., Abdou, D. Antimicrobial and Anti-Biofilm Activities of Bee Venom against some Dental pathogens. EKB Journal Management System, 2022; 60(3): 1-16. doi: 10.21608/ejaps.2022.142059.1035

Antimicrobial and Anti-Biofilm Activities of Bee Venom against some Dental pathogens

Egyptian Journal of Pure and Applied Science
Article 1, Volume 60, Issue 3, October 2022, Page 1-16  XML PDF (1.18 MB)
Document Type: Original Article
DOI: 10.21608/ejaps.2022.142059.1035
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Authors
Haitham Mostafa email orcid 1; Saad Masryorcid 2; Mohamed Abou-Zeidorcid 3; Abeer Abd El Wahaborcid 1; Maha El Demellawyorcid 1; Dalia Abdou3
1Medical Biotechnology Department, Genetic Engineering Institute, City of Scientific Research and Technological applications, Borg Al Arab, Alexandria
2Department of Plant Protection and Biomolecular Diagnosis, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological applications, New Borg El-Arab City, Alexandria, Egypt.
3Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Abstract
Dental plaque is considered a poly-microbial biofilm in the oral cavity and is responsible for the most prevalent human infections. Plaque contains a variety of microorganisms embedded in an extracellular matrix of polymers that can also develop between the teeth and the gingival crevice making it even more difficult to remove; antibiotics cannot penetrate and reach the site of infection. The virulence of oral microbial biofilms is normally limited to the oral cavity. However, when these microbes or their components enter the connective tissues or circulation, they may increase the risk for some systemic diseases, such as cardiovascular diseases, diabetes, and immune-dysfunctional diseases. Bee venom (BV) has anti-inflammatory, anti-apoptotic, anti-fibrotic, anti-atherosclerotic, antibacterial, antiviral, antifungal, and anticancer effects.
Our study was conducted on 39 bacterial isolate samples out of 150 oral swab samples. Bee venom was analyzed by HPLC. Melittin and phospholipase A2 was used to generate a standard curve. The three most effective venoms on Streptococcus sp. give a mean inhibition zone of 40 %, 41.7 %, and 41.7 % at the concentration of 50 µg/ml. Pearson correlation between inhibition zones caused by the groups of bee venoms on the different identified isolates showed a non-significant positive relationship (P-value > 0.05) between all groups. However, bee venom can be used with other mouth-wash components to remove oral bacterial biofilms caused by streptococcus sp.
Keywords
Plaque; biofilm; bee venom; melittin; phospholipase A2
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