Enhanced production of xylanase enzyme by Fusariummoniliforme using submerged fermentation
Hussein, M., Hussein, M., Kheiralla, Z., Ahmed, H., El-Gendy, N. (2018). Enhanced production of xylanase enzyme by Fusariummoniliforme using submerged fermentation. EKB Journal Management System, 35(part 1), 227-236. doi: 10.21608/jsrs.2018.26739
M. Hussein; M. M. Hussein; Z. H. Kheiralla; H. A. Ahmed; N. Sh. El-Gendy. "Enhanced production of xylanase enzyme by Fusariummoniliforme using submerged fermentation". EKB Journal Management System, 35, part 1, 2018, 227-236. doi: 10.21608/jsrs.2018.26739
Hussein, M., Hussein, M., Kheiralla, Z., Ahmed, H., El-Gendy, N. (2018). 'Enhanced production of xylanase enzyme by Fusariummoniliforme using submerged fermentation', EKB Journal Management System, 35(part 1), pp. 227-236. doi: 10.21608/jsrs.2018.26739
Hussein, M., Hussein, M., Kheiralla, Z., Ahmed, H., El-Gendy, N. Enhanced production of xylanase enzyme by Fusariummoniliforme using submerged fermentation. EKB Journal Management System, 2018; 35(part 1): 227-236. doi: 10.21608/jsrs.2018.26739

Enhanced production of xylanase enzyme by Fusariummoniliforme using submerged fermentation

Journal of Scientific Research in Science
Article 30, Volume 35, part 1, August 2018, Page 227-236  XML PDF (671.23 K)
Document Type: Original Article
DOI: 10.21608/jsrs.2018.26739
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Authors
M. Hussein1; M. M. Hussein email 1; Z. H. Kheiralla2; H. A. Ahmed2; N. Sh. El-Gendy3
1Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Giza, Egypt.
2Botany Department,Faculty of Women for Arts, Science and Education, Ai Shams University.
3Petroleum Biotechnology lab, Department of Processes Design & Development,Egyptian Petroleum Research Institute, Nasr City, Cairo, Egypt
Abstract
Hemicellulose degrading enzymes play an important role in bioconversion of agro and agro-industrial wastes. In this study, production of hemicellulase by six fungal isolates was determined under submerged culture using corn cobs xylan as a carbon source and enzyme inducer at different incubation periods. The results indicated that Alternariatenuisshowed the lowest enzyme productivity (156.95 ± 2.07U/l)while the highest enzyme production (2,594.44 ± 62.25U/l) was found by Fusariummoniliforme. One-factor-at-a-time (OFAT) revealed maximum enzyme productivity of 10,950.11 ± 98.45 U/l at; corn cobs xylan (6g/l), yeast extract (4g/l), inorganic salts (MgSO4, KH2PO4, CaCl2, FeSO4, and MnSO4), initial pH (5), initial inoculum size (4%), 150 rpm and temperature (30 ).
 
Keywords
xylanase; Fusariummoniliforme; Submerged fermentation; corn cob xylan
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