Enhanced production of xylanase enzyme by Fusariummoniliforme using submerged fermentation | ||
Journal of Scientific Research in Science | ||
Article 30, Volume 35, part 1, August 2018, Pages 227-236 PDF (671.23 K) | ||
Document Type: Original Article | ||
DOI: 10.21608/jsrs.2018.26739 | ||
Authors | ||
M. Hussein1; M. M. Hussein* 1; Z. H. Kheiralla2; H. A. Ahmed2; N. Sh. El-Gendy3 | ||
1Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Giza, Egypt. | ||
2Botany Department,Faculty of Women for Arts, Science and Education, Ai Shams University. | ||
3Petroleum Biotechnology lab, Department of Processes Design & Development,Egyptian Petroleum Research Institute, Nasr City, Cairo, Egypt | ||
Abstract | ||
Hemicellulose degrading enzymes play an important role in bioconversion of agro and agro-industrial wastes. In this study, production of hemicellulase by six fungal isolates was determined under submerged culture using corn cobs xylan as a carbon source and enzyme inducer at different incubation periods. The results indicated that Alternariatenuisshowed the lowest enzyme productivity (156.95 ± 2.07U/l)while the highest enzyme production (2,594.44 ± 62.25U/l) was found by Fusariummoniliforme. One-factor-at-a-time (OFAT) revealed maximum enzyme productivity of 10,950.11 ± 98.45 U/l at; corn cobs xylan (6g/l), yeast extract (4g/l), inorganic salts (MgSO4, KH2PO4, CaCl2, FeSO4, and MnSO4), initial pH (5), initial inoculum size (4%), 150 rpm and temperature (30 ). | ||
Keywords | ||
xylanase; Fusariummoniliforme; Submerged fermentation; corn cob xylan | ||
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