Cytochrome C Oxidase Subunit-1(COX1) Gene in Tilapia Oreochromis Niloticus: Its Cloning and Characterization
Abumourad, I., Meselhy, S., Nie, P. (2011). Cytochrome C Oxidase Subunit-1(COX1) Gene in Tilapia Oreochromis Niloticus: Its Cloning and Characterization. EKB Journal Management System, 4(1), 12-17. doi: 10.21608/maj.2011.2676
Iman Abumourad; Salah Meselhy; Pin Nie. "Cytochrome C Oxidase Subunit-1(COX1) Gene in Tilapia Oreochromis Niloticus: Its Cloning and Characterization". EKB Journal Management System, 4, 1, 2011, 12-17. doi: 10.21608/maj.2011.2676
Abumourad, I., Meselhy, S., Nie, P. (2011). 'Cytochrome C Oxidase Subunit-1(COX1) Gene in Tilapia Oreochromis Niloticus: Its Cloning and Characterization', EKB Journal Management System, 4(1), pp. 12-17. doi: 10.21608/maj.2011.2676
Abumourad, I., Meselhy, S., Nie, P. Cytochrome C Oxidase Subunit-1(COX1) Gene in Tilapia Oreochromis Niloticus: Its Cloning and Characterization. EKB Journal Management System, 2011; 4(1): 12-17. doi: 10.21608/maj.2011.2676

Cytochrome C Oxidase Subunit-1(COX1) Gene in Tilapia Oreochromis Niloticus: Its Cloning and Characterization

Mediterranean Aquaculture Journal
Article 3, Volume 4, Issue 1, January 2011, Pages 12-17    PDF (1001.84 K)
Document Type: Original Article
DOI: 10.21608/maj.2011.2676
Authors
Iman Abumourad1; Salah Meselhy2; Pin Nie3
1Department of Hydrobiology, National Research Center
2Fish Health Department the WorldFish Center, Cairo, Egypt and
3State Key Laboratory of Freashwater Ecology and Biotechnology and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Hubei Province, China.
Abstract
The current study aimed to identify cytochrome c oxidase subunit 1 (ONCOX1) in Tilapia (Oreochromis niloticus) immunized by formalin-killed Flavobacterium columnarae. Suppressive subtractive hybridization (SSH) was utilized to construct a cDNA library and semi-quantitative RT-PCR analysis used to examine Oreochromis niloticus cytochrome c oxidase subunit 1 (COX1) gene expression. COX1 cDNA is composed of 1139 bps with a 1107 bps open reading frame, the predicted gene product is 369 amino acids with molecular weight of 40.16 kDa. The amino acid sequence revealed high identity with subunit 1 from Oreochromis mossambicus. Compared to ß-actin, the semi-quantitative RT-PCR revealed that ONCOX1 expressed in tissues of stimulated fish as up-regulated gene suggesting that this member of COX genes is probably involved in the general immune response against the pathogenic bacteria.
Keywords
tilapia; Cytochrome C oxidase; Protein prediction; Gene characterization; Tissue expression
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