SIMULTANEOUS DETERMINATION OF LEVAMISOLE AND OXYCLOZANIDE IN THE PHARMACEUTICAL PREPARATION BY CAPILLARY ELECTROPHORESIS | ||
| Al-Azhar Journal of Pharmaceutical Sciences | ||
| Article 8, Volume 66, Issue 2, September and October 2022, Pages 138-147 PDF (339.54 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajps.2022.269245 | ||
| Authors | ||
| Ahmed Mohamed Ahmed Ahmed* 1; Mohamed Nassar2; Ahmed El-Olemy2; Mohamed Emara2 | ||
| 1Higher Committee of Medical Specialists Ministry of Health and Population, Giza, Egypt. | ||
| 2Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751, Cairo, Egypt. | ||
| Abstract | ||
| A sensitive, precise and accurate capillary electrophoresis method was developed for simultaneous determination of levamisole and oxyclozanide in pure sample and pharmaceutical preparation. Capillary electrophoresis was presented as a simple separation analytical method for the simultaneous analysis of the deliberated drugs within a shorter analytical run time. In this study, separation was achieved on fused silica capillary (30 cm - 50 µm internal diameter); background electrolyte solution consisted of phosphate buffer (40 mM, pH 7.9) and UV detection at 227 nm. The method showed to be linear (r2 > 0.9998), precise (RSD <0.193%), accurate (recovery of 99.95% for levamisole and 100.12% for oxyclozanide), specific and robust. LOD and LOQ values were 0.099 µg mL-1 and 0.299 µg mL-1 respectively for levamisole and 0.075 µg mL-1 and 0.228 µg mL-1 respectively for oxyclozanide. The proposed method obtained well separation and had a perfect accuracy. The method was validated according to ICH guidelines and carried out for determination of the cited drugs in their pharmaceutical preparation. | ||
| Keywords | ||
| Levamisole; Oxyclozanide; Capillary electrophoresis; Pharmaceutical preparation | ||
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