Detection of Carbapenem-Resistant Genes and Specific Biofilm Association Genes in K. Pneumoniae Isolated from Medical Samples | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 47, Volume 89, Issue 2, October 2022, Pages 6356-6360 PDF (584.17 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejhm.2022.269974 | ||
| Authors | ||
| Hussam Mahmood Hasan* ; Hameed M. Jasim; Ghada Mohammed Salih | ||
| Abstract | ||
| Background: Klebsiella pneumonia is a ubiquitous encapsulated bacterial pathogen which cause various types of infections, this ability comes from the resistance gene and virulence factor genes. Methodology: Fifty clinical isolates of Klebsiella pneumoniae was diagnosed and characterized according to their chemical and molecular characteristics, then genomic DNA was extracted from each bacterial isolate to detect carbapenem-resistant (NDM-1, and OXA-1), and biofilm association gene including (fimH, and mrkD) by amplification of these genes using specific primers. Results: Results showed that NDM-1 and OXA-1 were found to be frequent in bacterial isolates in a percentage of 54% and 28% respectively. On the other hand, it was found that fimH is the most frequent in bacterial isolates (100%), while mrkD was found in a percentage of 86% of the total isolates. Conclusion: High prevalence of biofilm association genes and presence of carbapenem-resistant in varied isolates of K. pneumoniae pose an important public health thing. | ||
| Keywords | ||
| Klebsiella pneumoniae; Antibiotic resistance genes; Virulence genes | ||
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