Antibiofilm Activity of Fructophilic Lactic Acid Bacteria filtrate against Klebsiella Pneumoniae Virulence Gene Expression | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 58, Volume 89, Issue 2, October 2022, Pages 6438-6444 PDF (805.27 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejhm.2022.270278 | ||
| Authors | ||
| Hussam Mahmood Hasan* ; Ghada Mohammed Salih; Hameed M. Jasim | ||
| Abstract | ||
| Background: Fructophilic lactic acid bacteria (FLAB) are a group of LAB with unique growth features and are regarded as potential bioactive compound manufacturers. FLAB is discovered in D- fructose rich niches that favors D-fructose as a growth substrate triumphs over D-glucose. On D-glucose, they require electron acceptors to proliferate. The organisms have similar metabolic processes for carbohydrates. Fructobacillus spp < /em>. are Lactobacillus kunkeei, are members of this unique group. Methodology: Inhibitory effect of filtrate from four FLAB isolates from honeybee was detected against the growth of pathogenic Klebsiella pneumoniae isolates. Biofilm formation, and the expression of fimH, mrkD, and NDM-1, and OXA-1 virulence genes were also tested. Results: It was found that FLAB has antibacterial and antibiofilm effect. The incubation of K. pneumoniae with FLAB suspension at different three-time intervals (3, 6, and 9 hrs.) decreased expression of fimH, mrkD, NDM-1, and OXA-1virulence genes compared with the expression of the same genes in the absence of FLAB. Conclusion: all FLAB filtrate isolates tested, K. pneumoniae fimH, mrkD, NDM-1, and OXA-1virulence gene expression was down-regulated, when compared to the control culture. | ||
| Keywords | ||
| Fructophilic lactic acid bacteria; Klebsiella pneumoniae; Virulence genes; Gene expression; Antibacterial activity | ||
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