Bio- and Phyto-chemical Study on Nannochloropsis oculata algal Extract Incorporated with Gold Nanoparticles, in Vitro Study | ||||
| Frontiers in Scientific Research and Technology | ||||
| Volume 5, Issue 1, March 2023 PDF (1.67 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/fsrt.2022.170128.1073 | ||||
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| Authors | ||||
Wael Mahmoud Aboulthana   1; Randa Abdel-Salam Bekheit2; Waleed Mohamed Serag2
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| 133 El Bohouth St. (Former El Tahrir St.), P.O. 12622, Dokki, Giza, Egypt | ||||
| 2Chemistry Department (Biochemistry Branch), Faculty of Science, Suez University, P.O. 43518, Suez, Egypt. | ||||
| Abstract | ||||
| Background: Nannochloropsis oculata is rich in various active phyto-constituents have high ability to diminish generation of free radicals. These components have high molecular weights. Therefore, they integrated with nanotechnology to facilitate their absorption and hence increase their bioavailability. The present experiment was designed to evaluate role of gold nanoparticles (Au-NPs) incorporation in ameliorating the biological efficiency of N. oculata algal extract. Materials and Methods: The ultraviolet-visible (UV-VIS) spectroscopy, X-Ray Diffraction (XRD) spectrum, dynamic light scattering (DLS) measurement and transmission electron microscope (TEM) were used for characterizing the biosynthesized Au-NPs. The major phyto-constituents and the in vitro biological activities (total antioxidant, free radicals scavenging, anti-diabetic and anti-Alzheimer's activities) were quantified in N. oculata algal extract. The cytotoxic activity against growth and progression of human colon carcinoma (CACO-2) and hepatocellular (HEPG-2) cells was assayed. Also, safety of the algal extract after incorporating Au-NPs was evaluated by determining values of the median lethal dose (LD50) and compared with that of the extract itself. Results: It was found that the in vitro biological efficacy (antioxidant, scavenging, anti-diabetic and anti-Alzheimer's activities) increased in N. oculata algal extract after incorporating Au-NPs and this was related to elevating concentrations of the active phyto-constituents. Moreover, it ameliorated the cytotoxic activity against CACO-2 and HEPG-2 cells. Moreover, gold algal nano-extract was orally safer as compared to native algal extract itself. Conclusion: Incorporation of Au-NPs enhanced the in vitro biological activities of N. oculata algal extract. | ||||
| Keywords | ||||
| Nannochloropsis oculata; Green Nanotechnology; Gold Nanoparticles; Biological Activity; Toxicity | ||||
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