Using Molecular Tools to Compare Various Concentration Protocols Prior to Detecting SARS-Cov-2 RNA in Wastewater
Abd-Elshafy, D., Nadeem, R., Bahgat, M. (2022). Using Molecular Tools to Compare Various Concentration Protocols Prior to Detecting SARS-Cov-2 RNA in Wastewater. EKB Journal Management System, 65(131), 1241-1248. doi: 10.21608/ejchem.2022.165190.7030
Dina Nadeem Abd-Elshafy; Rola Nadeem; Mahmoud Bahgat. "Using Molecular Tools to Compare Various Concentration Protocols Prior to Detecting SARS-Cov-2 RNA in Wastewater". EKB Journal Management System, 65, 131, 2022, 1241-1248. doi: 10.21608/ejchem.2022.165190.7030
Abd-Elshafy, D., Nadeem, R., Bahgat, M. (2022). 'Using Molecular Tools to Compare Various Concentration Protocols Prior to Detecting SARS-Cov-2 RNA in Wastewater', EKB Journal Management System, 65(131), pp. 1241-1248. doi: 10.21608/ejchem.2022.165190.7030
Abd-Elshafy, D., Nadeem, R., Bahgat, M. Using Molecular Tools to Compare Various Concentration Protocols Prior to Detecting SARS-Cov-2 RNA in Wastewater. EKB Journal Management System, 2022; 65(131): 1241-1248. doi: 10.21608/ejchem.2022.165190.7030

Using Molecular Tools to Compare Various Concentration Protocols Prior to Detecting SARS-Cov-2 RNA in Wastewater

Egyptian Journal of Chemistry
Volume 65, Issue 131, December 2022, Page 1241-1248  XML PDF (324.94 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.165190.7030
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Authors
Dina Nadeem Abd-Elshafy email orcid 1; Rola Nadeemorcid 2; Mahmoud Bahgatorcid 3
1Water pollution research department, institute of environmental research and climate change, National research center,giza,Egypt
2Therapeutic chemistry department, Institute of pharmaceutical and drug industry research, National research center, Giza, Egypt
3Therapeutic Chemistry Department, National Research Centre (NRC), 33 EL Bohouth St. (former El Tahrir St.),
Abstract
Background: Reports showed presence of SARS-CoV-2 genetic material in wastewater. Wastewater concentration methods are optimized for detection of non-enveloped viruses so need to be adopted for enveloped viruses and their genetic material. Methods: Conventional (cRT-PCR) and quantitative real time RT-PCR (qRT-PCR) were used as readouts to compare 4 water concentration methods namely, A) filtration on negatively charged membrane followed by extracting RNA from it, B) adsorbtion-elution method, C) flocculation with skimmed milk and D) polyethylene glycol precipitation, to detect SARS-CoV-2 RNA and 229E human coronavirus (229E-HCoV) as a model for spike-containing enveloped virus from fresh and wastewater. Results: On using cRT-PCR: recovery rate of SARS-CoV-2 RNA was better using method A then B for fresh water and method B then D for wastewater. 229E-HCoV recovery from fresh water was better using method C then A and methods B then D for wastewater. On using qRT-PCR, both methods A and B were better for SARS-CoV-2 RNA recovery from both fresh and wastewater. For the 229E-HCoV methods A was the most efficient for fresh water and method B for wastewater. Conclusion: Method B is recommended for SARS-CoV-2 RNA or whole 229E-HCoV recovery from wastewater.
Keywords
SARS-CoV-2; Human corona virus 229E; molecular tools; wastewater; Concentration methods
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