Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR
Taha, M., Shouib, S., ELmonir, W., Zahra, S., Hegazy, E. (2023). Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR. EKB Journal Management System, 4(1), 168-181. doi: 10.21608/mid.2022.175107.1414
Marwa S. Taha; Sarah M. Shouib; Walid ELmonir; Shaima W. Zahra; Eman E. Hegazy. "Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR". EKB Journal Management System, 4, 1, 2023, 168-181. doi: 10.21608/mid.2022.175107.1414
Taha, M., Shouib, S., ELmonir, W., Zahra, S., Hegazy, E. (2023). 'Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR', EKB Journal Management System, 4(1), pp. 168-181. doi: 10.21608/mid.2022.175107.1414
Taha, M., Shouib, S., ELmonir, W., Zahra, S., Hegazy, E. Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR. EKB Journal Management System, 2023; 4(1): 168-181. doi: 10.21608/mid.2022.175107.1414

Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR

Microbes and Infectious Diseases
Article 21, Volume 4, Issue 1, February 2023, Page 168-181  XML PDF (667.3 K)
Document Type: Original Article
DOI: 10.21608/mid.2022.175107.1414
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Authors
Marwa S. Taha email orcid 1; Sarah M. Shouiborcid 2; Walid ELmonir3; Shaima W. Zahra4; Eman E. Hegazyorcid 1
1Department of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt.
2Department of Clinical Pathology, Faculty of Medicine, Tanta University, Egypt.
3Department of Hygiene and Preventive Medicine (Zoonoses), Faculty of Veterinary Medicine, Kafr Elsheikh University, Egypt.
4Department of Anesthesiology, Surgical Intensive Care and Pain Medicine, Faculty of Medicine, Tanta University, Egypt.
Abstract
Background: There has been an expansion in antibiotic resistance among Enterobacteriaceae over the previous ten years raising serious questions about global health. Our objectives were to isolate the carbapenem-resistant Gram-negative bacilli that cause blood stream infections and investigate the genetic relatedness between carbapenemase producingKlebsiella pneumoniae (K. pneumoniae) isolates. Methods: A total of 255 blood specimens were withdrawn into blood culture bottles and incubated, pathogenic microorganisms were identified, antibiotic susceptibility was done for Gram-negative isolates. Carbapenemase producers were screened for molecular detection of carbapenemase and extended spectrum β-lactamase genes. Results: One hundred and thirty-three (133) pathogens were isolated, of these 77 Gram-negative isolates (including 32 K. pneumoniae,18 E. coli, 12 Acinetobacter, 9 Pseudomonas and 6 Enterobacter cloacae). Out of them, 55 were carbapenem resistant by phenotypic method. 23/55 of them were harboring carbapenemase genes by PCR ERIC, results showed that Anesthesia intensive care unit (ICU) isolates showed >90% genetic similarity while isolates from Chest and Internal medicine ICUs showed <70% similarity. Conclusion: Increased awareness, persistent observation, and strict adherence to the recommendations for antibiotic stewardship should be used to limit spread of carbapenem resistant Gram-negative bacilli.
Keywords
BSIs; K. pneumoniae; ERIC PCR; Carbapenem resistant gram-negative bacilli
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